8WLL

Cryo-EM structure of human VMAT2 Y422C, in the presence of reserpine, determined in an inward-facing conformation

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Synaptic vesicular amine transporter	polymer	497	53763.2	1	UniProt (Q05940) Pfam (PF07690)	Homo sapiens (human)	Monoamine transporter,Solute carrier family 18 member 2,Vesicular amine transporter 2,VAT2
2	B (A)	reserpine	non-polymer		608.7	1	Chemie (YHR)

Sequence modifications

A: 1 - 474 (UniProt: Q05940)

PDB	External Database	Details
Cys 422	Tyr 422	engineered mutation
Ser 475	-	expression tag
Arg 476	-	expression tag
Leu 477	-	expression tag
Glu 478	-	expression tag
Glu 479	-	expression tag
Glu 480	-	expression tag
Leu 481	-	expression tag
Arg 482	-	expression tag
Arg 483	-	expression tag
Arg 484	-	expression tag
Thr 485	-	expression tag
Glu 486	-	expression tag
Gly 487	-	expression tag
Gly 488	-	expression tag
Ser 489	-	expression tag
Ser 490	-	expression tag
Asp 491	-	expression tag
Leu 492	-	expression tag
Glu 493	-	expression tag
Val 494	-	expression tag
Leu 495	-	expression tag
Phe 496	-	expression tag
Gln 497	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	53763.2
Non-Polymers*	Number of molecules	1
	Total formula weight	608.7
All*	Total formula weight	54371.8

*Water molecules are not included.