8WIH
Crystal structure of E. coli ThrS catalytic domain mutant G463A in complex with ATP
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B (A, B) | Threonine--tRNA ligase | polymer | 410 | 47941.5 | 2 | UniProt (A0A8S7FUD7) Pfam (PF00587) Pfam (PF03129) UniProt (by SIFTS) (P0A8M3) | Escherichia coli | Threonyl-tRNA synthetase,ThrRS |
| 2 | C, E (A, B) | ADENOSINE-5'-TRIPHOSPHATE | non-polymer | 507.2 | 2 | Chemie (ATP) | |||
| 3 | D, F (A, B) | ZINC ION | non-polymer | 65.4 | 2 | Chemie (ZN) | |||
| 4 | G, H (A, B) | water | water | 18.0 | 48 | Chemie (HOH) |
Sequence modifications
A, B: 242 - 642 (UniProt: A0A8S7FUD7)
| PDB | External Database | Details |
|---|---|---|
| Met 241 | - | initiating methionine |
| Ala 463 | Gly 463 | engineered mutation |
| Leu 643 | - | expression tag |
| Glu 644 | - | expression tag |
| His 645 | - | expression tag |
| His 646 | - | expression tag |
| His 647 | - | expression tag |
| His 648 | - | expression tag |
| His 649 | - | expression tag |
| His 650 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 2 |
| Total formula weight | 95883.1 | |
| Non-Polymers* | Number of molecules | 4 |
| Total formula weight | 1145.2 | |
| All* | Total formula weight | 97028.3 |
