8WIG

Crystal structure of E. coli ThrS catalytic domain mutant G463S/Q484A

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Threonine--tRNA ligase	polymer	410	47900.5	2	UniProt (A0A8S7FUD7) Pfam (PF00587) Pfam (PF03129) UniProt (by SIFTS) (P0A8M3)	Escherichia coli	Threonyl-tRNA synthetase,ThrRS
2	C, D (A, B)	ZINC ION	non-polymer		65.4	2	Chemie (ZN)

Sequence modifications

A, B: 242 - 642 (UniProt: A0A8S7FUD7)

PDB	External Database	Details
Met 241	-	initiating methionine
Ser 463	Gly 463	engineered mutation
Ala 484	Gln 484	engineered mutation
Leu 643	-	expression tag
Glu 644	-	expression tag
His 645	-	expression tag
His 646	-	expression tag
His 647	-	expression tag
His 648	-	expression tag
His 649	-	expression tag
His 650	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	95801.0
Non-Polymers*	Number of molecules	2
	Total formula weight	130.8
All*	Total formula weight	95931.8

*Water molecules are not included.