8WIF

Cryo- EM structure of Mycobacterium smegmatis 30S ribosomal subunit (body 2) of 70S ribosome and RafH.

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	a	16S rRNA	polymer	1528	495373.7	1	GenBank (118168627)	Mycolicibacterium smegmatis MC2 155
2	v	30S ribosomal protein S22	polymer	33	4164.3	1	UniProt (A0QR10) Pfam (PF08213) In PDB	Mycolicibacterium smegmatis MC2 155
3	d	30S ribosomal protein S3	polymer	275	30191.2	1	UniProt (A0QSD7) Pfam (PF07650) Pfam (PF00189) In PDB	Mycolicibacterium smegmatis MC2 155
4	e	30S ribosomal protein S4	polymer	201	23415.8	1	UniProt (A0A8B4QH64) Pfam (PF00163) Pfam (PF01479) UniProt (by SIFTS) (A0QSL7) In PDB	Mycolicibacterium smegmatis MC2 155
5	f	30S ribosomal protein S5	polymer	214	21946.1	1	UniProt (A0QSG6) Pfam (PF00333) Pfam (PF03719) In PDB	Mycolicibacterium smegmatis MC2 155
6	g	30S ribosomal protein S6	polymer	96	10991.6	1	UniProt (A0A8B4QKV6) Pfam (PF01250) UniProt (by SIFTS) (A0R7F9) In PDB	Mycolicibacterium smegmatis MC2 155
7	h	30S ribosomal protein S7	polymer	156	17660.4	1	UniProt (A0QS97) Pfam (PF00177) In PDB	Mycolicibacterium smegmatis MC2 155
8	i	30S ribosomal protein S8	polymer	132	14492.6	1	UniProt (A0QSG3) Pfam (PF00410) In PDB	Mycolicibacterium smegmatis MC2 155
9	j	30S ribosomal protein S9	polymer	150	16794.4	1	UniProt (A0QSP9) Pfam (PF00380) In PDB	Mycolicibacterium smegmatis MC2 155
10	k	30S ribosomal protein S10	polymer	101	11454.3	1	UniProt (A0QSD0) Pfam (PF00338) In PDB	Mycolicibacterium smegmatis MC2 155
11	l	30S ribosomal protein S11	polymer	138	14671.8	1	UniProt (A0QSL6) Pfam (PF00411) In PDB	Mycolicibacterium smegmatis MC2 155
12	m	30S ribosomal protein S12	polymer	124	13896.4	1	UniProt (A0QS96) Pfam (PF00164) In PDB	Mycolicibacterium smegmatis MC2 155
13	n	30S ribosomal protein S13	polymer	124	14249.6	1	UniProt (A0QSL5) Pfam (PF00416) In PDB	Mycolicibacterium smegmatis MC2 155
14	o	30S ribosomal protein S14A	polymer	101	11792.7	1	UniProt (A0R550) Pfam (PF00253) In PDB	Mycolicibacterium smegmatis MC2 155	30S ribosomal protein S14
15	p	30S ribosomal protein S15	polymer	89	10368.1	1	UniProt (A0QVQ3) Pfam (PF00312) In PDB	Mycolicibacterium smegmatis MC2 155
16	q	30S ribosomal protein S16	polymer	156	16795.2	1	UniProt (A0QV37) Pfam (PF00886) In PDB	Mycolicibacterium smegmatis MC2 155
17	r	30S ribosomal protein S17	polymer	98	11127.0	1	UniProt (A0QSE0) Pfam (PF00366) In PDB	Mycolicibacterium smegmatis MC2 155
18	s	30S ribosomal protein S18B	polymer	84	9524.2	1	UniProt (A0R7F7) Pfam (PF01084) In PDB	Mycolicibacterium smegmatis MC2 155
19	t	30S ribosomal protein S19	polymer	93	10800.6	1	UniProt (A0QSD5) Pfam (PF00203) In PDB	Mycolicibacterium smegmatis MC2 155
20	u	30S ribosomal protein S20	polymer	86	9556.1	1	UniProt (A0R102) Pfam (PF01649) In PDB	Mycolicibacterium smegmatis MC2 155
21	c	30S ribosomal protein S2	polymer	277	30145.2	1	UniProt (A0QVB8) Pfam (PF00318) In PDB	Mycolicibacterium smegmatis MC2 155
22	x	50S ribosomal protein L31	polymer	75	8312.5	1	UniProt (A0R215) Pfam (PF01197) In PDB	Mycolicibacterium smegmatis MC2 155
23	w	Ribosome hibernation promotion factor RafH	polymer	264	29898.9	1	UniProt (A0QZ86) Pfam (PF02482) Pfam (PF16321) In PDB	Mycolicibacterium smegmatis MC2 155

Sequence modifications

w: 1 - 258 (UniProt: A0QZ86)

PDB	External Database	Details
His 259	-	expression tag
His 260	-	expression tag
His 261	-	expression tag
His 262	-	expression tag
His 263	-	expression tag
His 264	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	23
	Total formula weight	837622.7
All*	Total formula weight	837622.7

*Water molecules are not included.