8WG8
Cryo-EM structures of peptide free and Gs-coupled GCGR
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | A | Guanine nucleotide-binding protein G(s) subunit alpha isoforms short | polymer | 361 | 41793.4 | 1 | Pfam (PF00503) UniProt (by SIFTS) (P63092) UniProt (by SIFTS) (P63096) In PDB | Homo sapiens (human) | |
2 | B | Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 | polymer | 371 | 40227.0 | 1 | UniProt (P54311) Pfam (PF00400) In PDB | Rattus norvegicus (Rat) | Transducin beta chain 1 |
3 | G | Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 | polymer | 71 | 7861.1 | 1 | UniProt (P63212) Pfam (PF00631) In PDB | Bos taurus (Bovine) | G gamma-I |
4 | N | Nanobody-35 | polymer | 140 | 15343.0 | 1 | synthetic construct | ||
5 | R | Glucagon receptor | polymer | 407 | 46812.4 | 1 | UniProt (P47871) Pfam (PF02793) Pfam (PF00002) In PDB | Homo sapiens (human) | GL-R |
6 | E | PRO-PRO-PRO-PRO-PHE-SER-ASN-LEU-VAL-MET-ASP-ASP-LEU-LYS-ASN-LYS-LYS | polymer | 17 | 1943.3 | 1 | Spodoptera frugiperda |
Sequence modifications
B: 2 - 340 (UniProt: P54311)
PDB | External Database | Details |
---|---|---|
Met -4 | - | initiating methionine |
Gly -3 | - | expression tag |
Ser -2 | - | expression tag |
Leu -1 | - | expression tag |
Leu 0 | - | expression tag |
Gln 1 | - | expression tag |
Gly 341 | - | expression tag |
Ser 342 | - | expression tag |
Ser 343 | - | expression tag |
Gly 344 | - | expression tag |
Gly 345 | - | expression tag |
Gly 346 | - | expression tag |
Gly 347 | - | expression tag |
Ser 348 | - | expression tag |
Gly 349 | - | expression tag |
Gly 350 | - | expression tag |
Gly 351 | - | expression tag |
Gly 352 | - | expression tag |
Ser 353 | - | expression tag |
Ser 354 | - | expression tag |
Gly 355 | - | expression tag |
Val 356 | - | expression tag |
Ser 357 | - | expression tag |
Gly 358 | - | expression tag |
Trp 359 | - | expression tag |
Arg 360 | - | expression tag |
Leu 361 | - | expression tag |
Phe 362 | - | expression tag |
Lys 363 | - | expression tag |
Lys 364 | - | expression tag |
Ile 365 | - | expression tag |
Ser 366 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 6 |
Total formula weight | 153980.3 | |
All* | Total formula weight | 153980.3 |