8WEH

Crystal structure of Brassica napus MIK2 ectodomain (N393A mutant)

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	MALE DISCOVERER 1-INTERACTING RECEPTOR-LIKE KINASE 2	polymer	672	74187.6	1	UniProt (A0A816I5A6) Pfam (PF08263) Pfam (PF13855) Pfam (PF00560)	Brassica napus (rape)
2	B, F (B, F)	beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		586.5	2	In PDB GlyTouCan (G15407YE)
3	C, E (C, E)	2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		424.4	2	In PDB GlyTouCan (G42666HT)
4	D (D)	beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-[alpha-L-fucopyranose-(1-6)]2-acetamido-2-deoxy-beta-D-glucopyranose	branched		732.7	1	In PDB GlyTouCan (G32152BH)
5	G (G)	2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-[alpha-L-fucopyranose-(1-6)]2-acetamido-2-deoxy-beta-D-glucopyranose	branched		570.5	1	In PDB GlyTouCan (G21290RB)
6	H, I (A)	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	2	Chemie (NAG)

Sequence modifications

A: 31 - 690 (UniProt: A0A816I5A6)

PDB	External Database	Details
Ala 393	Asn 393	engineered mutation
Ser 546	Thr 546	conflict
Glu 691	-	expression tag
Phe 692	-	expression tag
His 693	-	expression tag
His 694	-	expression tag
His 695	-	expression tag
His 696	-	expression tag
His 697	-	expression tag
His 698	-	expression tag
His 699	-	expression tag
His 700	-	expression tag
His 701	-	expression tag
His 702	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	74187.6
Branched	Number of molecules	6
	Total formula weight	3325.1
Non-Polymers*	Number of molecules	2
	Total formula weight	442.4
All*	Total formula weight	77955.1

*Water molecules are not included.