8WEE

Crystal structure of Arabidopsis thaliana MIK2 ectodomain in complex with SCOOP12

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	MDIS1-interacting receptor like kinase 2	polymer	712	78853.9	1	UniProt (Q8VZG8) Pfam (PF08263) Pfam (PF00560) Pfam (PF13855)	Arabidopsis thaliana (Mouse-ear cress)	AtMIK2,Probable LRR receptor-like serine/threonine-protein kinase At4g08850
2	B (B)	SERINE-RICH ENDOGENOUS PEPTIDE (SCOOP)	polymer	13	1318.4	1	UniProt (by SIFTS) (B3H7I1)	Arabidopsis thaliana
3	C, E, F, G, H (C, E, F, G, H)	2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		424.4	5	In PDB GlyTouCan (G42666HT)
4	D (D)	2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-[alpha-L-fucopyranose-(1-6)]2-acetamido-2-deoxy-beta-D-glucopyranose	branched		570.5	1	In PDB GlyTouCan (G21290RB)
5	I, J, K, L, M... (A)	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	7	Chemie (NAG)
6	P, Q (A, B)	water	water		18.0	613	Chemie (HOH)

Sequence modifications

A: 1 - 700 (UniProt: Q8VZG8)

PDB	External Database	Details
Glu 137	Leu 137	engineered mutation
Lys 164	Asp 164	engineered mutation
Phe 564	Ser 564	engineered mutation
Glu 701	-	expression tag
Phe 702	-	expression tag
His 703	-	expression tag
His 704	-	expression tag
His 705	-	expression tag
His 706	-	expression tag
His 707	-	expression tag
His 708	-	expression tag
His 709	-	expression tag
His 710	-	expression tag
His 711	-	expression tag
His 712	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	80172.3
Branched	Number of molecules	6
	Total formula weight	2692.5
Non-Polymers*	Number of molecules	7
	Total formula weight	1548.5
All*	Total formula weight	84413.3

*Water molecules are not included.