8WED
Crystal structure of Arabidopsis thaliana MIK2 ectodomain in complex with BAK1 ectodomain and Fusarium oxysporum SCOOPL
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, D (A, D) | MDIS1-interacting receptor like kinase 2 | polymer | 683 | 75533.3 | 2 | UniProt (Q8VZG8) Pfam (PF08263) Pfam (PF00560) Pfam (PF13855) | Arabidopsis thaliana (Mouse-ear cress) | AtMIK2,Probable LRR receptor-like serine/threonine-protein kinase At4g08850 |
| 2 | B, E (B, E) | BRASSINOSTEROID INSENSITIVE 1-associated receptor kinase 1 | polymer | 230 | 25183.8 | 2 | UniProt (Q94F62) Pfam (PF08263) Pfam (PF13855) Pfam (PF00560) | Arabidopsis thaliana (Mouse-ear cress) | AtBAK1,BRI1-associated receptor kinase 1,Protein ELONGATED,Somatic embryogenesis receptor kinase 3,AtSERK3,Somatic embryogenesis receptor-like kinase 3 |
| 3 | C, F (C, F) | a SCOOP-like peptide from F. oxysporum f. sp. conglutinans strain Fo5176 | polymer | 13 | 1349.3 | 2 | Fusarium oxysporum f. sp. pisi HDV247 | ||
| 4 | G (G) | alpha-L-fucopyranose-(1-3)-[2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)][alpha-L-fucopyranose-(1-6)]2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 716.7 | 1 | In PDB GlyTouCan (G63564LA) | |||
| 5 | H, I, J, K, P... (H, I, J, K, P...) | 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 424.4 | 8 | In PDB GlyTouCan (G42666HT) | |||
| 6 | L (L) | 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-[alpha-L-fucopyranose-(1-6)]2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 570.5 | 1 | In PDB GlyTouCan (G21290RB) | |||
| 7 | M, N (M, N) | beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 586.5 | 2 | In PDB GlyTouCan (G15407YE) | |||
| 8 | O (O) | beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-[alpha-L-fucopyranose-(1-6)]2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 732.7 | 1 | In PDB GlyTouCan (G32152BH) | |||
| 9 | S, T (S, T) | alpha-L-fucopyranose-(1-6)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 367.3 | 2 | In PDB GlyTouCan (G86851RC) | |||
| 10 | AA, BA, CA, DA, V... (D, A) | 2-acetamido-2-deoxy-beta-D-glucopyranose | non-polymer | 221.2 | 9 | Chemie (NAG) |
Sequence modifications
A, D: 1 - 683 (UniProt: Q8VZG8)
B, E: 1 - 220 (UniProt: Q94F62)
| PDB | External Database | Details |
|---|---|---|
| Glu 137 | Leu 137 | engineered mutation |
| Lys 164 | Asp 164 | engineered mutation |
| Phe 564 | Ser 564 | engineered mutation |
| PDB | External Database | Details |
|---|---|---|
| His 221 | - | expression tag |
| His 222 | - | expression tag |
| His 223 | - | expression tag |
| His 224 | - | expression tag |
| His 225 | - | expression tag |
| His 226 | - | expression tag |
| His 227 | - | expression tag |
| His 228 | - | expression tag |
| His 229 | - | expression tag |
| His 230 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 6 |
| Total formula weight | 204132.8 | |
| Branched | Number of molecules | 15 |
| Total formula weight | 7322.9 | |
| Non-Polymers* | Number of molecules | 9 |
| Total formula weight | 1990.9 | |
| All* | Total formula weight | 213446.6 |
