8WCX

Cryo-EM structure of MsRv1273c/72c from Mycobacterium smegmatis in the AMPPNP-bound IFasym-1 state

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Transmembrane ATP-binding protein ABC transporter	polymer	584	63010.5	1	UniProt (I7FIY9) Pfam (PF00664) Pfam (PF00005) UniProt (by SIFTS) (P9WQJ1)	Mycolicibacterium smegmatis MC2 155
2	B (B)	ABC transporter transmembrane region	polymer	643	70310.3	1	UniProt (I7GDB1) Pfam (PF00664) Pfam (PF00005) UniProt (by SIFTS) (P9WQJ3)	Mycolicibacterium smegmatis MC2 155
3	C, E (A, B)	PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER	non-polymer		506.2	2	Chemie (ANP)
4	D, F (A, B)	MAGNESIUM ION	non-polymer		24.3	2	Chemie (MG)

Sequence modifications

A: 1 - 578 (UniProt: I7FIY9)

PDB	External Database	Details
Ser -5	-	expression tag
Tyr -4	-	expression tag
Phe -3	-	expression tag
Gln -2	-	expression tag
Ser -1	-	expression tag
Asn 0	-	expression tag

B: 1 - 625 (UniProt: I7GDB1)

PDB	External Database	Details
His 626	-	linker
Leu 627	-	linker
Gly 628	-	linker
Gly 629	-	linker
Ile 630	-	linker
Lys 631	-	linker
Ala 632	-	linker
Phe 633	-	linker
His 634	-	expression tag
His 635	-	expression tag
His 636	-	expression tag
His 637	-	expression tag
His 638	-	expression tag
His 639	-	expression tag
His 640	-	expression tag
His 641	-	expression tag
His 642	-	expression tag
His 643	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	133320.8
Non-Polymers*	Number of molecules	4
	Total formula weight	1061.0
All*	Total formula weight	134381.8

*Water molecules are not included.