8WBO

Crystal structure of cis-Epoxysuccinate Hydrolases RhCESH[L] mutant D18N complexed with sulfate ions

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Epoxide hydrolase	polymer	264	29350.9	1	UniProt (Q1KLR5) Pfam (PF00702)	Rhodococcus opacus
2	B, C (A)	SULFATE ION	non-polymer		96.1	2	Chemie (SO4)
3	D (A)	water	water		18.0	251	Chemie (HOH)

Sequence modifications

A: 11 - 253 (UniProt: Q1KLR5)

PDB	External Database	Details
Met -10	-	initiating methionine
Gly -9	-	expression tag
Ser -8	-	expression tag
Ser -7	-	expression tag
His -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
His -3	-	expression tag
His -2	-	expression tag
His -1	-	expression tag
Ser 0	-	expression tag
Ser 1	-	expression tag
Gly 2	-	expression tag
Leu 3	-	expression tag
Val 4	-	expression tag
Pro 5	-	expression tag
Arg 6	-	expression tag
Gly 7	-	expression tag
Ser 8	-	expression tag
His 9	-	expression tag
Met 10	-	expression tag
Asn 18	Asp 18	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	29350.9
Non-Polymers*	Number of molecules	2
	Total formula weight	192.1
All*	Total formula weight	29543.0

*Water molecules are not included.