8WAM

Cryo-EM structure of the ABCG25 E232Q mutant bound to ATP and Magnesium

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	ABC transporter G family member 25	polymer	698	77120.2	2	UniProt (Q84TH5) Pfam (PF00005) Pfam (PF19055) Pfam (PF01061) In PDB	Arabidopsis thaliana (thale cress)
2	A, B	ADENOSINE-5'-TRIPHOSPHATE	non-polymer		507.2	2	Chemie (ATP)
3	A, B	MAGNESIUM ION	non-polymer		24.3	2	Chemie (MG)

Sequence modifications

A, B: 1 - 662 (UniProt: Q84TH5)

PDB	External Database	Details
Met -35	-	initiating methionine
Asp -34	-	expression tag
Tyr -33	-	expression tag
Lys -32	-	expression tag
Asp -31	-	expression tag
His -30	-	expression tag
Asp -29	-	expression tag
Gly -28	-	expression tag
Asp -27	-	expression tag
Tyr -26	-	expression tag
Lys -25	-	expression tag
Asp -24	-	expression tag
His -23	-	expression tag
Asp -22	-	expression tag
Ile -21	-	expression tag
Asp -20	-	expression tag
Tyr -19	-	expression tag
Lys -18	-	expression tag
Asp -17	-	expression tag
Asp -16	-	expression tag
Asp -15	-	expression tag
Asp -14	-	expression tag
Lys -13	-	expression tag
Gly -12	-	expression tag
Ser -11	-	expression tag
Asp -10	-	expression tag
Leu -9	-	expression tag
Glu -8	-	expression tag
Val -7	-	expression tag
Leu -6	-	expression tag
Phe -5	-	expression tag
Gln -4	-	expression tag
Gly -3	-	expression tag
Pro -2	-	expression tag
Gly -1	-	expression tag
Ser 0	-	expression tag
Gln 232	Glu 232	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	154240.3
Non-Polymers*	Number of molecules	4
	Total formula weight	1063.0
All*	Total formula weight	155303.3

*Water molecules are not included.