8WA3
Cryo-EM structure of peptide free and Gs-coupled GIPR
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (R) | Gastric inhibitory polypeptide receptor,Fusion protein | polymer | 573 | 64950.7 | 1 | UniProt (P48546) Pfam (PF02793) Pfam (PF00002) | Homo sapiens (human) | GIP-R,Glucose-dependent insulinotropic polypeptide receptor |
| 2 | B (A) | Guanine nucleotide-binding protein G(s) subunit alpha isoforms short | polymer | 394 | 45743.4 | 1 | UniProt (P04896) Pfam (PF00503) | Bos taurus (cattle) | Adenylate cyclase-stimulating G alpha protein |
| 3 | C (B) | Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1,O-antigen polymerase | polymer | 371 | 40227.0 | 1 | UniProt (P54311) UniProt (A0A0P6XLS5) Pfam (PF00400) | Rattus norvegicus (Norway rat) | Transducin beta chain 1 |
| 4 | D (G) | Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 | polymer | 71 | 7861.1 | 1 | UniProt (P63212) Pfam (PF00631) | Bos taurus (cattle) | G gamma-I |
| 5 | E (N) | Nanobody-35 | polymer | 140 | 15343.0 | 1 | synthetic construct |
Sequence modifications
R: 22 - 421 (UniProt: P48546)
R: 437 - 594 (PDB: 8WA3)
A: 1 - 394 (UniProt: P04896)
B: 2 - 340 (UniProt: P54311)
B: 359 - 366 (UniProt: A0A0P6XLS5)
| PDB | External Database | Details |
|---|---|---|
| Phe 345 | Thr 345 | engineered mutation |
| Gly 422 | - | linker |
| Ser 423 | - | linker |
| Ser 424 | - | linker |
| Gly 425 | - | linker |
| Gly 426 | - | linker |
| Gly 427 | - | linker |
| Gly 428 | - | linker |
| Ser 429 | - | linker |
| Gly 430 | - | linker |
| Gly 431 | - | linker |
| Gly 432 | - | linker |
| Gly 433 | - | linker |
| Ser 434 | - | linker |
| Ser 435 | - | linker |
| Gly 436 | - | linker |
A: 1 - 394 (UniProt: P04896)
| PDB | External Database | Details |
|---|---|---|
| Asn 54 | Ser 54 | engineered mutation |
| Ala 226 | Gly 226 | engineered mutation |
| Ala 268 | Glu 268 | engineered mutation |
| Lys 271 | Asn 271 | engineered mutation |
| Asp 274 | Lys 274 | engineered mutation |
| Lys 280 | Arg 280 | engineered mutation |
| Asp 284 | Thr 284 | engineered mutation |
| Thr 285 | Ile 285 | engineered mutation |
| Ser 366 | Ala 366 | engineered mutation |
| PDB | External Database | Details |
|---|---|---|
| Met -4 | - | initiating methionine |
| Gly -3 | - | expression tag |
| Ser -2 | - | expression tag |
| Leu -1 | - | expression tag |
| Leu 0 | - | expression tag |
| Gln 1 | - | expression tag |
| Gly 341 | - | linker |
| Ser 342 | - | linker |
| Ser 343 | - | linker |
| Gly 344 | - | linker |
| Gly 345 | - | linker |
| Gly 346 | - | linker |
| Gly 347 | - | linker |
| Ser 348 | - | linker |
| Gly 349 | - | linker |
| Gly 350 | - | linker |
| Gly 351 | - | linker |
| Gly 352 | - | linker |
| Ser 353 | - | linker |
| Ser 354 | - | linker |
| Gly 355 | - | linker |
| Val 356 | - | linker |
| Ser 357 | - | linker |
| Gly 358 | - | linker |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 5 |
| Total formula weight | 174125.3 | |
| All* | Total formula weight | 174125.3 |
