8W9U

Crystal structure of CspR from Bacillus subtilis complexed with SAH

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Putative tRNA (cytidine(34)-2'-O)-methyltransferase	polymer	190	21770.5	2	UniProt (O31590) Pfam (PF00588)	Bacillus subtilis subsp. subtilis str. 168	tRNA (cytidine/uridine-2'-O-)-methyltransferase CspR
2	C, D, G, H (A, B)	SULFATE ION	non-polymer		96.1	4	Chemie (SO4)
3	E, I (A, B)	S-ADENOSYL-L-HOMOCYSTEINE	non-polymer		384.4	2	Chemie (SAH)
4	F (A)	GLYCEROL	non-polymer		92.1	1	Chemie (GOL)
5	J, K (A, B)	water	water		18.0	89	Chemie (HOH)

Sequence modifications

A, B: 2 - 160 (UniProt: O31590)

PDB	External Database	Details
Met -29	-	initiating methionine
Ala -28	-	expression tag
Gly -27	-	expression tag
Ser -26	-	expression tag
His -25	-	expression tag
His -24	-	expression tag
His -23	-	expression tag
His -22	-	expression tag
His -21	-	expression tag
His -20	-	expression tag
Gly -19	-	expression tag
Met -18	-	expression tag
Ala -17	-	expression tag
Ser -16	-	expression tag
Met -15	-	expression tag
Thr -14	-	expression tag
Gly -13	-	expression tag
Gly -12	-	expression tag
Gln -11	-	expression tag
Gln -10	-	expression tag
Met -9	-	expression tag
Gly -8	-	expression tag
Arg -7	-	expression tag
Ser -6	-	expression tag
Gly -5	-	expression tag
Asp -4	-	expression tag
Asp -3	-	expression tag
Asp -2	-	expression tag
Asp -1	-	expression tag
Lys 0	-	expression tag
Val 1	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	43541.1
Non-Polymers*	Number of molecules	7
	Total formula weight	1245.2
All*	Total formula weight	44786.2

*Water molecules are not included.