8W9M

Cryo-EM structure of the cyanobacterial nitrate transporter NrtBCD in complex with ATP

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (B, A)	Nitrate transport permease protein	polymer	279	30483.1	2	UniProt (Q8YZ77)	Nostoc sp. PCC 7120 = FACHB-418	ABC nitrate transport permease protein
2	C (C)	Nitrate transport ATP-binding protein	polymer	682	76325.2	1	UniProt (Q8YZ76)	Nostoc sp. PCC 7120 = FACHB-418
3	D (D)	Nitrate transport ATP-binding protein	polymer	277	31398.2	1	UniProt (Q8YZ75)	Nostoc sp. PCC 7120 = FACHB-418
4	E, G (C, D)	ADENOSINE-5'-TRIPHOSPHATE	non-polymer		507.2	2	Chemie (ATP)
5	F, H (C, D)	MAGNESIUM ION	non-polymer		24.3	2	Chemie (MG)

Sequence modifications

C: 1 - 657 (UniProt: Q8YZ76)

PDB	External Database	Details
Gln 164	Glu 164	engineered mutation
Ser 658	-	expression tag
Ser 659	-	expression tag
Gly 660	-	expression tag
Asp 661	-	expression tag
Tyr 662	-	expression tag
Lys 663	-	expression tag
Asp 664	-	expression tag
His 665	-	expression tag
Asp 666	-	expression tag
Gly 667	-	expression tag
Asp 668	-	expression tag
Tyr 669	-	expression tag
Lys 670	-	expression tag
Asp 671	-	expression tag
His 672	-	expression tag
Asp 673	-	expression tag
Ile 674	-	expression tag
Asp 675	-	expression tag
Tyr 676	-	expression tag
Lys 677	-	expression tag
Asp 678	-	expression tag
Asp 679	-	expression tag
Asp 680	-	expression tag
Asp 681	-	expression tag
Lys 682	-	expression tag

D: 1 - 277 (UniProt: Q8YZ75)

PDB	External Database	Details
Gln 179	Glu 179	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	168689.4
Non-Polymers*	Number of molecules	4
	Total formula weight	1063.0
All*	Total formula weight	169752.4

*Water molecules are not included.