8W9D

Cryo-EM structure of the Rpd3S-nucleosome complex from budding yeast in State 1

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Transcriptional regulatory protein SIN3	polymer	1536	175047.3	1	UniProt (P22579) Pfam (PF02671) Pfam (PF08295) Pfam (PF16879)	Saccharomyces cerevisiae (baker's yeast)
2	B, F (E, F)	Transcriptional regulatory protein RCO1	polymer	684	78951.3	2	UniProt (Q04779) Pfam (PF00628)	Saccharomyces cerevisiae (baker's yeast)
3	C (B)	Histone deacetylase RPD3	polymer	433	48962.0	1	UniProt (P32561) Pfam (PF00850)	Saccharomyces cerevisiae (baker's yeast)
4	D, E, Q (C, D, G)	Chromatin modification-related protein EAF3	polymer	401	45266.4	3	UniProt (Q12432) Pfam (PF11717) Pfam (PF22732) Pfam (PF05712)	Saccharomyces cerevisiae (baker's yeast)	ESA1-associated factor 3
5	G, K (a, e)	Histone H3.1	polymer	136	15437.2	2	UniProt (P68431) Pfam (PF00125)	Homo sapiens (human)
6	H, L (b, f)	Histone H4	polymer	103	11394.4	2	UniProt (P62805) Pfam (PF15511)	Homo sapiens (human)
7	I, M (c, g)	Histone H2A type 1-B/E	polymer	130	14165.6	2	UniProt (P04908) Pfam (PF00125) Pfam (PF16211)	Homo sapiens (human)
8	J, N (d, h)	Histone H2B type 1-K	polymer	126	13921.2	2	UniProt (O60814) Pfam (PF00125)	Homo sapiens (human)
9	O (i)	5-DNA	polymer	147	45368.1	1		Homo sapiens
10	P (j)	3-DNA	polymer	147	45359.0	1		Homo sapiens
11	R (H)	Unclear peptide	polymer	5	487.6	1		Saccharomyces cerevisiae
12	AA, S, T, U, V... (F, E, B)	ZINC ION	non-polymer		65.4	7	Chemie (ZN)
13	X, Y (B)	POTASSIUM ION	non-polymer		39.1	2	Chemie (K)

Contents of the asymmetric unit

Polymers	Number of chains	18
	Total formula weight	718762.5
Non-Polymers*	Number of molecules	9
	Total formula weight	536.1
All*	Total formula weight	719298.5

*Water molecules are not included.