8W8N
Thermus thermophilus initiation transcription complex in the pre-translocated state
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | A, B | DNA-directed RNA polymerase subunit alpha | polymer | 315 | 35056.2 | 2 | UniProt (Q5SHR6) Pfam (PF01193) Pfam (PF01000) Pfam (PF03118) In PDB | Thermus thermophilus HB8 | RNAP subunit alpha,RNA polymerase subunit alpha,Transcriptase subunit alpha |
2 | C | DNA-directed RNA polymerase subunit beta | polymer | 1119 | 125436.5 | 1 | UniProt (Q8RQE9) Pfam (PF04563) Pfam (PF04561) Pfam (PF04565) Pfam (PF10385) Pfam (PF00562) Pfam (PF04560) In PDB | Thermus thermophilus HB8 | RNAP subunit beta,RNA polymerase subunit beta,Transcriptase subunit beta |
3 | D | DNA-directed RNA polymerase subunit beta' | polymer | 1524 | 170997.4 | 1 | UniProt (Q8RQE8) Pfam (PF04997) Pfam (PF21668) Pfam (PF00623) Pfam (PF04983) Pfam (PF05000) Pfam (PF04998) In PDB | Thermus thermophilus HB8 | RNAP subunit beta',RNA polymerase subunit beta',Transcriptase subunit beta' |
4 | E | DNA-directed RNA polymerase subunit omega | polymer | 99 | 11533.3 | 1 | UniProt (Q8RQE7) Pfam (PF01192) In PDB | Thermus thermophilus HB8 | RNAP omega subunit,RNA polymerase omega subunit,Transcriptase subunit omega |
5 | F | RNA polymerase sigma factor SigA | polymer | 443 | 50769.4 | 1 | UniProt (Q5SKW1) Pfam (PF00140) Pfam (PF04542) Pfam (PF04539) Pfam (PF04545) In PDB | Thermus thermophilus HB8 | |
6 | G | DNA (21-MER) | polymer | 21 | 6409.1 | 1 | synthetic construct | ||
7 | H | DNA (27-MER) | polymer | 27 | 8421.4 | 1 | synthetic construct | ||
8 | I | RNA (5'-D(*PP*PP*P)-R(*GP*GP*U)-3') | polymer | 3 | 1111.6 | 1 | synthetic construct | ||
9 | B, D, F | MAGNESIUM ION | non-polymer | 24.3 | 6 | Chemie (MG) | |||
10 | D | ZINC ION | non-polymer | 65.4 | 2 | Chemie (ZN) | |||
11 | water | water | 18.0 | 140 | Chemie (HOH) |
Sequence modifications
F: 1 - 423 (UniProt: Q5SKW1)
PDB | External Database | Details |
---|---|---|
Met -19 | - | expression tag |
Gly -18 | - | expression tag |
Ser -17 | - | expression tag |
Ser -16 | - | expression tag |
His -15 | - | expression tag |
His -14 | - | expression tag |
His -13 | - | expression tag |
His -12 | - | expression tag |
His -11 | - | expression tag |
His -10 | - | expression tag |
Ser -9 | - | expression tag |
Ser -8 | - | expression tag |
Gly -7 | - | expression tag |
Leu -6 | - | expression tag |
Val -5 | - | expression tag |
Pro -4 | - | expression tag |
Arg -3 | - | expression tag |
Gly -2 | - | expression tag |
Ser -1 | - | expression tag |
His 0 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 9 |
Total formula weight | 444791.1 | |
Non-Polymers* | Number of molecules | 8 |
Total formula weight | 276.6 | |
All* | Total formula weight | 445067.8 |