8W7R

H. walsbyi bacteriorhodopsin mutant - W94F

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Bacteriorhodopsin-I	polymer	262	28451.1	2	UniProt (Q18DH8) Pfam (PF01036)	Haloquadratum walsbyi DSM 16790	HwBR,Squarebop I
2	C, L (A, B)	GLYCEROL	non-polymer		92.1	2	Chemie (GOL)
3	D, I, J, K (A, B)	PENTAETHYLENE GLYCOL	non-polymer		238.3	4	Chemie (1PE)
4	E, G, H (A, B)	(2R)-2,3-dihydroxypropyl (9Z)-octadec-9-enoate	non-polymer		356.5	3	Chemie (OLC)
5	F, M (A, B)	RETINAL	non-polymer		284.4	2	Chemie (RET)
6	N, O (A, B)	water	water		18.0	26	Chemie (HOH)

Sequence modifications

A, B: 3 - 254 (UniProt: Q18DH8)

PDB	External Database	Details
Met 1	-	initiating methionine
Ala 2	-	expression tag
Phe 94	Trp 94	engineered mutation
Leu 255	-	expression tag
Glu 256	-	expression tag
His 257	-	expression tag
His 258	-	expression tag
His 259	-	expression tag
His 260	-	expression tag
His 261	-	expression tag
His 262	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	56902.1
Non-Polymers*	Number of molecules	11
	Total formula weight	2775.8
All*	Total formula weight	59677.9

*Water molecules are not included.