8W22

Umb1 umbrella toxin particle (local refinement of UmbB1 bound ALF of UmbC1 and UmbA1)

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Intein C-terminal splicing domain-containing protein	polymer	1368	142716.7	1	UniProt (Q9ACV2) Pfam (PF03752) Pfam (PF14428) Pfam (PF07591) In PDB	Streptomyces coelicolor A3(2)
2	B	Secreted protein	polymer	166	17186.3	1	UniProt (Q9ACV3) In PDB	Streptomyces coelicolor A3(2)
3	C	Secreted esterase	polymer	515	54437.1	1	UniProt (Q9ACV4) Pfam (PF00089) Pfam (PF13517) In PDB	Streptomyces coelicolor A3(2)

Sequence modifications

A: 33 - 1368 (UniProt: Q9ACV2)

PDB	External Database	Details
Met 1	-	initiating methionine
Arg 2	-	expression tag
Arg 3	-	expression tag
Arg 4	-	expression tag
Ile 5	-	expression tag
Pro 6	-	expression tag
Ser 7	-	expression tag
Arg 8	-	expression tag
Thr 9	-	expression tag
Pro 10	-	expression tag
Gly 11	-	expression tag
Ser 12	-	expression tag
Gly 13	-	expression tag
Ala 14	-	expression tag
Lys 15	-	expression tag
Gln 16	-	expression tag
Lys 17	-	expression tag
Ser 18	-	expression tag
Trp 19	-	expression tag
Phe 20	-	expression tag
Pro 21	-	expression tag
Arg 22	-	expression tag
Arg 23	-	expression tag
Ser 24	-	expression tag
Leu 25	-	expression tag
Gln 26	-	expression tag
Val 27	-	expression tag
Leu 28	-	expression tag
Leu 29	-	expression tag
Ser 30	-	expression tag
Ala 31	-	expression tag
Gly 32	-	expression tag

C: 1 - 505 (UniProt: Q9ACV4)

PDB	External Database	Details
Gly 506	-	expression tag
Ser 507	-	expression tag
His 508	-	expression tag
His 509	-	expression tag
His 510	-	expression tag
His 511	-	expression tag
His 512	-	expression tag
His 513	-	expression tag
His 514	-	expression tag
His 515	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	3
	Total formula weight	214340.1
All*	Total formula weight	214340.1

*Water molecules are not included.