8W0I

Cryo-EM structure of the human MCM2-7 heterohexamer

This is a non-PDB format compatible entry.

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (2)	DNA replication licensing factor MCM2	polymer	904	102034.1	1	UniProt (P49736) Pfam (PF12619) Pfam (PF14551) Pfam (PF17207) Pfam (PF00493) Pfam (PF17855) Pfam (PF23669)	Homo sapiens (human)	Minichromosome maintenance protein 2 homolog,Nuclear protein BM28
2	B (3)	DNA replication licensing factor MCM3	polymer	810	91251.9	1	UniProt (P25205) Pfam (PF17855) Pfam (PF23191) Pfam (PF14551) Pfam (PF17207) Pfam (PF00493)	Homo sapiens (human)	DNA polymerase alpha holoenzyme-associated protein P1,P1-MCM3,RLF subunit beta,p102
3	C (4)	DNA replication licensing factor MCM4	polymer	866	96975.1	1	UniProt (P33991) Pfam (PF14551) Pfam (PF17207) Pfam (PF00493) Pfam (PF17855) Pfam (PF21128)	Homo sapiens (human)	CDC21 homolog,P1-CDC21
4	D (5)	DNA replication licensing factor MCM5	polymer	734	82406.6	1	UniProt (P33992) Pfam (PF14551) Pfam (PF17207) Pfam (PF00493) Pfam (PF17855) Pfam (PF21933)	Homo sapiens (human)	CDC46 homolog,P1-CDC46
5	E (6)	DNA replication licensing factor MCM6	polymer	821	93010.3	1	UniProt (Q14566) Pfam (PF14551) Pfam (PF17207) Pfam (PF00493) Pfam (PF17855) Pfam (PF18263)	Homo sapiens (human)	p105MCM
6	F (7)	DNA replication licensing factor MCM7	polymer	719	81411.9	1	UniProt (P33993) Pfam (PF14551) Pfam (PF17207) Pfam (PF00493) Pfam (PF17855) Pfam (PF24901)	Homo sapiens (human)	CDC47 homolog,P1.1-MCM3
7	G, L, O, P, S (2, 4, 5, 6, 7)	ZINC ION	non-polymer		65.4	5	Chemie (ZN)
8	H, J, M, Q, T (2, 3, 4, 6, 7)	MAGNESIUM ION	non-polymer		24.3	5	Chemie (MG)
9	I, N, R, U (2, 4, 6, 7)	ADENOSINE-5'-TRIPHOSPHATE	non-polymer		507.2	4	Chemie (ATP)
10	K (3)	ADENOSINE-5'-DIPHOSPHATE	non-polymer		427.2	1	Chemie (ADP)

Sequence modifications

3: 2 - 808 (UniProt: P25205)

PDB	External Database	Details
Ser -1	-	expression tag
Asn 0	-	expression tag
Ala 1	-	expression tag

4: 1 - 863 (UniProt: P33991)

PDB	External Database	Details
Ser -2	-	expression tag
Asn -1	-	expression tag
Ala 0	-	expression tag
Met 650	Leu 650	variant

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	547089.9
Non-Polymers*	Number of molecules	15
	Total formula weight	2904.5
All*	Total formula weight	549994.4

*Water molecules are not included.