8VY7

CryoEM structure of Gi-coupled TAS2R14 with cholesterol and an intracellular tastant

This is a non-PDB format compatible entry.

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Guanine nucleotide-binding protein G(i) subunit alpha-1	polymer	354	40414.0	1	UniProt (P63096) Pfam (PF00503) In PDB	Homo sapiens (human)	Adenylate cyclase-inhibiting G alpha protein
2	B	Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	polymer	401	42805.5	1	UniProt (P62873) Pfam (PF00400) In PDB	Homo sapiens (human)	Transducin beta chain 1
3	C	Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	polymer	71	7861.1	1	UniProt (P59768) Pfam (PF00631) In PDB	Homo sapiens (human)	G gamma-I
4	D	scFv16	polymer	267	28668.9	1		Mus musculus (mouse)
5	R	Taste receptor type 2 member 14	polymer	637	70959.8	1	UniProt (Q9NYV8) Pfam (PF05296) In PDB	Homo sapiens (human)	T2R14,Taste receptor family B member 1,TRB1
6	R	4-methyl-N-[(2M)-2-(1H-tetrazol-5-yl)phenyl]-6-(trifluoromethyl)pyrimidin-2-amine	non-polymer		321.3	1	Chemie (A1AEI)
7	R	CHOLESTEROL	non-polymer		386.7	1	Chemie (CLR)

Sequence modifications

A: 1 - 354 (UniProt: P63096)

PDB	External Database	Details
Asn 47	Ser 47	engineered mutation
Ala 203	Gly 203	engineered mutation
Ala 245	Glu 245	engineered mutation
Ser 326	Ala 326	engineered mutation

B: 2 - 340 (UniProt: P62873)

PDB	External Database	Details
His -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
Leu -10	-	expression tag
Glu -9	-	expression tag
Val -8	-	expression tag
Leu -7	-	expression tag
Phe -6	-	expression tag
Gln -5	-	expression tag
Gly -4	-	expression tag
Pro -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
Ser 0	-	expression tag
Gly 1	-	expression tag
Gly 341	-	expression tag
Gly 342	-	expression tag
Ser 343	-	expression tag
Gly 344	-	expression tag
Gly 345	-	expression tag
Gly 346	-	expression tag
Gly 347	-	expression tag
Ser 348	-	expression tag
Gly 349	-	expression tag
Gly 350	-	expression tag
Ser 351	-	expression tag
Ser 352	-	expression tag
Ser 353	-	expression tag
Gly 354	-	expression tag
Gly 355	-	expression tag
Gly 356	-	expression tag
Gly 357	-	expression tag
Ser 358	-	expression tag
Gly 359	-	expression tag
Gly 360	-	expression tag
Gly 361	-	expression tag
Gly 362	-	expression tag
Ser 363	-	expression tag
Gly 364	-	expression tag
Gly 365	-	expression tag
Ser 366	-	expression tag
Ser 367	-	expression tag
Ser 368	-	expression tag
Gly 369	-	expression tag
Gly 370	-	expression tag
Val 371	-	expression tag
Ser 372	-	expression tag
Gly 373	-	expression tag
Trp 374	-	expression tag
Arg 375	-	expression tag
Leu 376	-	expression tag
Phe 377	-	expression tag
Lys 378	-	expression tag
Lys 379	-	expression tag
Ile 380	-	expression tag
Ser 381	-	expression tag
Gly 382	-	expression tag
Gly 383	-	expression tag
Ser 384	-	expression tag

R: -150 - 0 (PDB: 8VY7)
R: 1 - 302 (UniProt: Q9NYV8)

PDB	External Database	Details
Arg 225	Lys 225	conflict

R: 303 - 486 (PDB: 8VY7)

Contents of the asymmetric unit

Polymers	Number of chains	5
	Total formula weight	190709.4
Non-Polymers*	Number of molecules	2
	Total formula weight	707.9
All*	Total formula weight	191417.3

*Water molecules are not included.