Summary Structural details Experimental details Functional details Sequence Neighbor History Downloads

8VVU

Anisomycin-bound mammalian ribosome with partially accommodated A-site tRNA

This is a non-PDB format compatible entry.

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	uL2	polymer	257	28088.9	1	UniProt (G1TT27) Pfam (PF00181) Pfam (PF03947)	Oryctolagus cuniculus (rabbit)
2	B (B)	uL3	polymer	403	46108.0	1	UniProt (G1TL06) Pfam (PF00297)	Oryctolagus cuniculus (rabbit)
3	C (C)	uL4	polymer	413	46388.9	1	UniProt (G1SVW5) Pfam (PF00573) Pfam (PF14374)	Oryctolagus cuniculus (rabbit)
4	D (D)	uL18	polymer	297	34481.8	1	UniProt (G1SYJ6) Pfam (PF17144) Pfam (PF14204) UniProt (by SIFTS) (P19949)	Oryctolagus cuniculus (rabbit)
5	E (E)	L6	polymer	291	33028.3	1	UniProt (G1SKF7) Pfam (PF03868) Pfam (PF01159)	Oryctolagus cuniculus (rabbit)
6	F (F)	uL30	polymer	249	29514.3	1	UniProt (G1SV32) Pfam (PF08079) Pfam (PF00327)	Oryctolagus cuniculus (rabbit)
7	G (G)	L7A	polymer	319	36267.6	1	UniProt (G1STW0) Pfam (PF01248)	Oryctolagus cuniculus (rabbit)
8	H (H)	L9	polymer	192	21871.4	1	UniProt (G1SWI6) Pfam (PF00347)	Oryctolagus cuniculus (rabbit)
9	I (I)	L10	polymer	214	24643.1	1	UniProt (B7NZQ2) Pfam (PF00252)	Oryctolagus cuniculus (rabbit)
10	J (J)	uL5	polymer	178	20288.5	1	UniProt (G1TUB8) Pfam (PF00281) Pfam (PF00673)	Oryctolagus cuniculus (rabbit)
11	K (K)	eL13	polymer	211	24331.7	1	UniProt (G1TPV0)	Oryctolagus cuniculus (rabbit)
12	L (L)	eL14	polymer	218	23870.5	1	UniProt (G1SZ12) Pfam (PF01929)	Oryctolagus cuniculus (rabbit)
13	M (M)	L15	polymer	204	24207.3	1	UniProt (G1T0C1) Pfam (PF00827)	Oryctolagus cuniculus (rabbit)
14	N (N)	uL13	polymer	203	23533.3	1		Oryctolagus cuniculus (rabbit)
15	O (O)	uL22	polymer	213	24938.4	1	UniProt (G1TVT6) Pfam (PF00237) UniProt (by SIFTS) (G1SCJ6)	Oryctolagus cuniculus (rabbit)	60S ribosomal protein L17
16	P (P)	eL18	polymer	188	21699.7	1	UniProt (G1TFE0) Pfam (PF17135)	Oryctolagus cuniculus (rabbit)
17	Q (Q)	eL19	polymer	212	25455.5	1		Oryctolagus cuniculus (rabbit)
18	R (R)	L18A	polymer	224	26103.4	1		Oryctolagus cuniculus (rabbit)
19	S (S)	eL21	polymer	160	18610.0	1	UniProt (G1SHQ2) Pfam (PF01157)	Oryctolagus cuniculus (rabbit)
20	T (T)	eL22	polymer	128	14813.0	1	UniProt (G1TSG1) Pfam (PF01776)	Oryctolagus cuniculus (rabbit)
21	U (U)	L23	polymer	140	14892.5	1	UniProt (G1T6D1) Pfam (PF00238)	Oryctolagus cuniculus (rabbit)
22	V (V)	uL24	polymer	157	17765.0	1	UniProt (G1SE28) Pfam (PF01246)	Oryctolagus cuniculus (rabbit)	60S ribosomal protein L24
23	W (W)	uL23	polymer	156	17768.2	1	UniProt (G1SE76) Pfam (PF03939) Pfam (PF00276)	Oryctolagus cuniculus (rabbit)
24	X (X)	L26	polymer	145	17303.4	1	UniProt (G1SQH0) Pfam (PF16906) Pfam (PF00467)	Oryctolagus cuniculus (rabbit)
25	Y (Y)	L27	polymer	136	15835.8	1	UniProt (G1TXF6) Pfam (PF00467) Pfam (PF01777)	Oryctolagus cuniculus (rabbit)
26	Z (Z)	L27A	polymer	148	16620.6	1	UniProt (G1SNY0) Pfam (PF00828)	Oryctolagus cuniculus (rabbit)
27	AA (AA)	L29	polymer	245	26708.7	1	UniProt (G1SGR6) Pfam (PF01779)	Oryctolagus cuniculus (rabbit)
28	BA (BA)	eL30	polymer	115	12807.1	1	UniProt (G1TDL2) Pfam (PF01248)	Oryctolagus cuniculus (rabbit)
29	CA (CA)	L31	polymer	125	14494.9	1	UniProt (G1SHG0) Pfam (PF01198)	Oryctolagus cuniculus (rabbit)
30	DA (DA)	L32	polymer	135	15898.9	1	UniProt (G1U437) Pfam (PF01655) UniProt (by SIFTS) (G1TUN8)	Oryctolagus cuniculus (rabbit)
31	EA (EA)	eL33	polymer	110	12580.8	1	UniProt (G1SF08) Pfam (PF01247)	Oryctolagus cuniculus (rabbit)
32	FA (FA)	L34	polymer	129	14557.5	1	UniProt (G1U945) Pfam (PF01199) UniProt (by SIFTS) (G1TXG5)	Oryctolagus cuniculus (rabbit)
33	GA (GA)	L35	polymer	123	14566.6	1	UniProt (G1SIT5) Pfam (PF00831)	Oryctolagus cuniculus (rabbit)
34	HA (HA)	L36	polymer	105	12263.8	1	UniProt (G1TTQ5) Pfam (PF01158)	Oryctolagus cuniculus (rabbit)
35	IA (IA)	L37	polymer	97	11111.0	1	UniProt (U3KPD5) Pfam (PF01907)	Oryctolagus cuniculus (rabbit)
36	JA (JA)	eL38	polymer	70	8238.9	1	UniProt (G1U001) Pfam (PF01781)	Oryctolagus cuniculus (rabbit)
37	KA (KA)	eL39	polymer	51	6455.8	1	UniProt (G1TTN1) Pfam (PF00832)	Oryctolagus cuniculus (rabbit)
38	LA (LA)	eL40	polymer	128	14695.3	1		Oryctolagus cuniculus (rabbit)
39	MA (MA)	eL41	polymer	25	3473.5	1	UniProt (A0A087WNH4) Pfam (PF05162)	Oryctolagus cuniculus (rabbit)	60s ribosomal protein l41
40	NA (NA)	eL42	polymer	106	12477.0	1	UniProt (G1U344) Pfam (PF00935) UniProt (by SIFTS) (G1T040)	Oryctolagus cuniculus (rabbit)
41	OA (OA)	eL43	polymer	92	10299.4	1	UniProt (G1SY53) Pfam (PF01780)	Oryctolagus cuniculus (rabbit)
42	PA (PA)	L28	polymer	137	15783.6	1	UniProt (G1U7L1) Pfam (PF01778)	Oryctolagus cuniculus (rabbit)
43	QA (RA)	L12	polymer	165	17847.6	1	UniProt (G1SMR7) Pfam (PF03946) Pfam (PF00298)	Oryctolagus cuniculus (rabbit)
44	RA (SA)	P-site tRNA	polymer	76	24505.7	1		Bos taurus (bovine)
45	SA (TA)	E-site tRNA	polymer	76	24431.5	1		Oryctolagus cuniculus (rabbit)
46	TA (VA)	mRNA	polymer	12	3734.2	1		Lampyridae
47	UA (WA)	28S rRNA	polymer	3584	1161601.0	1		Oryctolagus cuniculus (rabbit)
48	VA (XA)	5S rRNA	polymer	120	38691.9	1	GenBank (X06789.1)	Oryctolagus cuniculus (rabbit)
49	WA (YA)	5.8S rRNA	polymer	156	50143.6	1		Oryctolagus cuniculus (rabbit)
50	XA (ZA)	18S rRNA	polymer	1869	602777.9	1		Oryctolagus cuniculus (rabbit)
51	YA (AB)	RPSA	polymer	295	32928.0	1	UniProt (G1TLT8) Pfam (PF00318) Pfam (PF16122)	Oryctolagus cuniculus (rabbit)	40S ribosomal protein SA, 37 kDa laminin receptor precursor,37LRP,37/67 kDa laminin receptor,LRP/LR,67 kDa laminin receptor,67LR,Laminin receptor 1,LamR,Laminin-binding protein precursor p40,LBP/p40
52	ZA (BB)	S3A	polymer	264	30002.1	1	UniProt (G1SS70) Pfam (PF01015)	Oryctolagus cuniculus (rabbit)	40S ribosomal protein S3a
53	AB (CB)	S2-like	polymer	293	31327.4	1		Oryctolagus cuniculus (rabbit)
54	BB (DB)	S3	polymer	281	31146.6	1	UniProt (G1TNM3) Pfam (PF07650) Pfam (PF00189)	Oryctolagus cuniculus (rabbit)	Ribosomal protein S3
55	CB (EB)	S4	polymer	263	29654.9	1	UniProt (G1TK17) Pfam (PF08071) Pfam (PF00900) Pfam (PF00467) Pfam (PF16121)	Oryctolagus cuniculus (rabbit)	40S ribosomal protein S4
56	DB (FB)	S5	polymer	204	22913.5	1	UniProt (G1TFM5) Pfam (PF00177)	Oryctolagus cuniculus (rabbit)	40S ribosomal protein S5
57	EB (GB)	eS6	polymer	249	28751.9	1	UniProt (G1TM55) Pfam (PF01092)	Oryctolagus cuniculus (rabbit)	40S ribosomal protein S6
58	FB (HB)	eS7	polymer	432	47356.2	1	UniProt (G1SVB0) Pfam (PF01251)	Oryctolagus cuniculus (rabbit)	40S ribosomal protein S7
59	GB (IB)	S8	polymer	208	24263.4	1	UniProt (G1TJW1) Pfam (PF01201)	Oryctolagus cuniculus (rabbit)	40S ribosomal protein S8
60	HB (JB)	S9	polymer	194	22641.6	1	UniProt (B7NZS8) Pfam (PF00163) Pfam (PF01479)	Oryctolagus cuniculus (rabbit)	Ribosomal protein S9
61	IB (KB)	S10	polymer	165	18933.8	1	UniProt (G1TPV3) Pfam (PF03501)	Oryctolagus cuniculus (rabbit)
62	JB (LB)	S11	polymer	158	18468.8	1	UniProt (G1TRM4) Pfam (PF16205) Pfam (PF00366)	Oryctolagus cuniculus (rabbit)	40S ribosomal protein S11
63	KB (MB)	S12	polymer	132	14539.0	1	UniProt (G1SFR8) Pfam (PF01248)	Oryctolagus cuniculus (rabbit)	40S ribosomal protein S12
64	LB (NB)	uS15	polymer	151	17259.4	1	UniProt (G1SP51) Pfam (PF08069) Pfam (PF00312)	Oryctolagus cuniculus (rabbit)	Ribosomal protein S13
65	MB (OB)	S14	polymer	151	16302.8	1	UniProt (G1U472) Pfam (PF00411) UniProt (by SIFTS) (G1T1F0)	Oryctolagus cuniculus (rabbit)
66	NB (PB)	S15	polymer	145	17049.2	1	UniProt (G1U0Q2) Pfam (PF00203)	Oryctolagus cuniculus (rabbit)	40S ribosomal protein S15
67	OB (QB)	uS9	polymer	172	19213.5	1	UniProt (G1SGX4) Pfam (PF00380)	Oryctolagus cuniculus (rabbit)	Ribosomal protein S16
68	PB (RB)	eS17	polymer	135	15552.1	1	UniProt (G1TU13) Pfam (PF00833)	Oryctolagus cuniculus (rabbit)	40S ribosomal protein S17
69	QB (SB)	S18	polymer	152	17759.8	1	UniProt (G1TPG3) Pfam (PF00416)	Oryctolagus cuniculus (rabbit)	40S ribosomal protein S18
70	RB (TB)	S19	polymer	145	16106.6	1	UniProt (G1TN62) Pfam (PF01090)	Oryctolagus cuniculus (rabbit)
71	SB (UB)	uS10	polymer	119	13398.8	1	UniProt (G1SIZ2) Pfam (PF00338)	Oryctolagus cuniculus (rabbit)
72	TB (VB)	S21	polymer	83	9124.4	1	UniProt (G1TM82) Pfam (PF01249)	Oryctolagus cuniculus (rabbit)	40S ribosomal protein S21
73	UB (WB)	S15A	polymer	130	14865.6	1	UniProt (G1TG89) Pfam (PF00410)	Oryctolagus cuniculus (rabbit)	40S Ribosomal protein S15a
74	VB (XB)	S23	polymer	143	15844.7	1	UniProt (G1SZ47) Pfam (PF00164)	Oryctolagus cuniculus (rabbit)	40S ribosomal protein S23
75	WB (YB)	S24	polymer	131	15237.1	1	UniProt (G1T3D8) Pfam (PF01282)	Oryctolagus cuniculus (rabbit)	40S ribosomal protein S24
76	XB (ZB)	eS25	polymer	124	13645.0	1	UniProt (G1TDB3) Pfam (PF03297)	Oryctolagus cuniculus (rabbit)	40S ribosomal protein S25
77	YB (AC)	S26	polymer	115	13047.5	1	UniProt (G1TFE8) Pfam (PF01283)	Oryctolagus cuniculus (rabbit)
78	ZB (BC)	S27	polymer	84	9480.2	1	UniProt (G1TZ76) Pfam (PF01667)	Oryctolagus cuniculus (rabbit)	40S ribosomal protein S27
79	AC (CC)	S28	polymer	69	7855.1	1	UniProt (G1TIB4) Pfam (PF01200)	Oryctolagus cuniculus (rabbit)	40S Ribosomal protein S28
80	BC (DC)	uS14	polymer	56	6690.8	1	UniProt (G1U7M4) Pfam (PF00253)	Oryctolagus cuniculus (rabbit)	40S ribosomal protein S29
81	CC (EC)	S30	polymer	133	14498.9	1	UniProt (G1T8A2) Pfam (PF00240) Pfam (PF04758)	Oryctolagus cuniculus (rabbit)	40S ribosomal protein S30
82	DC (FC)	S27A	polymer	188	21431.7	1	UniProt (G1SK22) Pfam (PF00240) Pfam (PF01599)	Oryctolagus cuniculus (rabbit)	40S ribosomal protein S27a, Ubiquitin carboxyl extension protein 80,Ubiquitin-40S ribosomal protein S27a
83	EC (GC)	RACK1	polymer	317	35115.7	1	UniProt (G1SJB4) Pfam (PF00400)	Oryctolagus cuniculus (rabbit)
84	FC (IC)	nascent chain	polymer	4	489.5	1		Oryctolagus cuniculus (rabbit)
85	GC (b)	RPLP0	polymer	318	34380.5	1	UniProt (G1SPK4) Pfam (PF17777) Pfam (PF00428) Pfam (PF00466)	Oryctolagus cuniculus (rabbit)
86	HC (At)	A-site tRNA	polymer	74	23887.3	1		Bos taurus (bovine)
87	AD, AE, AF, AG, BD... (WA, ZA, I, FA, AC...)	MAGNESIUM ION	non-polymer		24.3	102	Chemie (MG)
88	JC, LC, MC, NC, OC... (FA, IA, LA, NA, OA...)	ZINC ION	non-polymer		65.4	8	Chemie (ZN)
89	OF (WA)	ANISOMYCIN	non-polymer		265.3	1	Chemie (ANM)
90	PF (WA)	SPERMIDINE	non-polymer		145.2	1	Chemie (SPD)
91	MG (ZA)	SERINE	non-polymer		105.1	1	Chemie (SER)

Sequence modifications

K: 1 - 211 (UniProt: G1TPV0)

PDB	External Database	Details
Ile 46	-	insertion
Ala 47	-	insertion
Pro 48	-	insertion
Arg 49	-	insertion
Pro 50	-	insertion
Ala 51	-	insertion
Ala 52	-	insertion
Gly 53	-	insertion
Pro 54	-	insertion

O: 1 - 213 (UniProt: G1TVT6)

PDB	External Database	Details
Ser 43	Ala 43	conflict

P: 1 - 188 (UniProt: G1TFE0)

PDB	External Database	Details
Asp 4	Asn 4	conflict
Arg 14	Trp 14	conflict
Met 53	Leu 53	conflict
Arg 58	Trp 58	conflict
Arg 75	Gln 75	conflict
Ala 80	Pro 80	conflict
Val 86	Ile 86	conflict
Arg 104	His 104	conflict
Arg 110	Cys 110	conflict
Val 137	Gly 137	conflict
Gly 157	Arg 157	conflict
Arg 181	Trp 181	conflict

T: 1 - 128 (UniProt: G1TSG1)

PDB	External Database	Details
Leu 18	Val 18	conflict
Gly 32	Arg 32	conflict
Ala 36	Glu 36	conflict
Phe 39	Ser 39	conflict
Gly 54	Arg 54	conflict
Val 60	Ala 60	conflict
Ser 62	Thr 62	conflict
Leu 63	Ile 63	conflict
Arg 97	His 97	conflict
Thr 106	Ser 106	conflict
Glu 126	Asp 126	conflict

V: 1 - 157 (UniProt: G1SE28)

PDB	External Database	Details
Ser 78	Phe 78	conflict

JA: 1 - 70 (UniProt: G1U001)

PDB	External Database	Details
Lys 24	Asn 24	conflict

XA: 1 - 120 (GenBank: X06789.1)

PDB	External Database	Details
U 2	N 2	conflict
C 36	N 36	conflict
U 102	N 102	conflict
U 112	N 112	conflict
U 114	N 114	conflict
U 119	C 119	conflict
U 120	N 120	conflict

EB: 1 - 263 (UniProt: G1TK17)

PDB	External Database	Details
Gly 25	Ser 25	conflict
Arg 51	Lys 51	conflict
Thr 78	Ala 78	conflict
Val 156	Met 156	conflict

IB: 1 - 208 (UniProt: G1TJW1)

PDB	External Database	Details
Arg 47	Gly 47	conflict

TB: 1 - 145 (UniProt: G1TN62)

PDB	External Database	Details
Gly 119	Trp 119	conflict

VB: 1 - 83 (UniProt: G1TM82)

PDB	External Database	Details
Asn 3	Ser 3	conflict
Asp 4	Asn 4	conflict
Gln 33	Pro 33	conflict
Phe 50	Ser 50	conflict
Ala 75	Ser 75	conflict
Asp 76	His 76	conflict
Lys 81	Gln 81	conflict

AC: 1 - 115 (UniProt: G1TFE8)

PDB	External Database	Details
Arg 28	Cys 28	conflict
Ala 56	Val 56	conflict
Arg 109	Pro 109	conflict

b: 1 - 318 (UniProt: G1SPK4)

PDB	External Database	Details
Leu 82	Ile 82	conflict

Sequence viewer

Contents of the asymmetric unit

Polymers	Number of chains	86
Polymers	Total formula weight	3499738.5
Non-Polymers*	Number of molecules	113
Non-Polymers*	Total formula weight	3518.0
All*	Total formula weight	3503256.5

*Water molecules are not included.

		Sequence (fasta)
		PDBx/mmCIF
		PDBML format (no-atom)
		Validation report (PDF)