8VQA

Prefusion stabilized structure of the SARS-CoV-2 fusion machinery

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C	Spike protein S2	polymer	624	68328.5	3	UniProt (P0DTC2) In PDB	Sarbecovirus
2	D, E, F, G, H...	2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		424.4	12	In PDB GlyTouCan (G42666HT)
3	A, B, C	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	6	Chemie (NAG)

Sequence modifications

A, B, C: 686 - 1208 (UniProt: P0DTC2)

PDB	External Database	Details
Met 654	-	initiating methionine
Gly 655	-	expression tag
Ile 656	-	expression tag
Leu 657	-	expression tag
Pro 658	-	expression tag
Ser 659	-	expression tag
Pro 660	-	expression tag
Gly 661	-	expression tag
Met 662	-	expression tag
Pro 663	-	expression tag
Ala 664	-	expression tag
Leu 665	-	expression tag
Leu 666	-	expression tag
Ser 667	-	expression tag
Leu 668	-	expression tag
Val 669	-	expression tag
Ser 670	-	expression tag
Leu 671	-	expression tag
Leu 672	-	expression tag
Ser 673	-	expression tag
Val 674	-	expression tag
Leu 675	-	expression tag
Leu 676	-	expression tag
Met 677	-	expression tag
Gly 678	-	expression tag
Cys 679	-	expression tag
Val 680	-	expression tag
Ala 681	-	expression tag
Glu 682	-	expression tag
Thr 683	-	expression tag
Gly 684	-	expression tag
Thr 685	-	expression tag
Cys 707	Tyr 707	conflict
Cys 883	Thr 883	conflict
Pro 888	Phe 888	conflict
Pro 892	Ala 892	conflict
Pro 893	Ala 893	conflict
Pro 899	Ala 899	conflict
Glu 907	Asn 907	conflict
Pro 942	Ala 942	conflict
Phe 961	Thr 961	conflict
Cys 970	Phe 970	conflict
Pro 987	Val 987	conflict
Gln 994	Asp 994	conflict
Gln 998	Thr 998	conflict
Cys 999	Gly 999	conflict
Met 1010	Gln 1010	conflict
Met 1011	Gln 1011	conflict
Tyr 1018	Ile 1018	conflict
Met 1023	Asn 1023	conflict
Gly 1209	-	expression tag
Ser 1210	-	expression tag
Gly 1211	-	expression tag
Tyr 1212	-	expression tag
Ile 1213	-	expression tag
Pro 1214	-	expression tag
Glu 1215	-	expression tag
Ala 1216	-	expression tag
Pro 1217	-	expression tag
Arg 1218	-	expression tag
Asp 1219	-	expression tag
Gly 1220	-	expression tag
Gln 1221	-	expression tag
Ala 1222	-	expression tag
Tyr 1223	-	expression tag
Val 1224	-	expression tag
Arg 1225	-	expression tag
Lys 1226	-	expression tag
Asp 1227	-	expression tag
Gly 1228	-	expression tag
Glu 1229	-	expression tag
Trp 1230	-	expression tag
Val 1231	-	expression tag
Leu 1232	-	expression tag
Leu 1233	-	expression tag
Ser 1234	-	expression tag
Thr 1235	-	expression tag
Phe 1236	-	expression tag
Leu 1237	-	expression tag
Gly 1238	-	expression tag
Arg 1239	-	expression tag
Ser 1240	-	expression tag
Leu 1241	-	expression tag
Glu 1242	-	expression tag
Val 1243	-	expression tag
Leu 1244	-	expression tag
Phe 1245	-	expression tag
Gln 1246	-	expression tag
Gly 1247	-	expression tag
Pro 1248	-	expression tag
Gly 1249	-	expression tag
Ser 1250	-	expression tag
Gly 1251	-	expression tag
Gly 1252	-	expression tag
Leu 1253	-	expression tag
Asn 1254	-	expression tag
Asp 1255	-	expression tag
Ile 1256	-	expression tag
Phe 1257	-	expression tag
Glu 1258	-	expression tag
Ala 1259	-	expression tag
Gln 1260	-	expression tag
Lys 1261	-	expression tag
Ile 1262	-	expression tag
Glu 1263	-	expression tag
Trp 1264	-	expression tag
His 1265	-	expression tag
Glu 1266	-	expression tag
Gly 1267	-	expression tag
Ser 1268	-	expression tag
Gly 1269	-	expression tag
His 1270	-	expression tag
His 1271	-	expression tag
His 1272	-	expression tag
His 1273	-	expression tag
His 1274	-	expression tag
His 1275	-	expression tag
His 1276	-	expression tag
His 1277	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	3
	Total formula weight	204985.5
Branched	Number of molecules	12
	Total formula weight	5092.8
Non-Polymers*	Number of molecules	6
	Total formula weight	1327.2
All*	Total formula weight	211405.5

*Water molecules are not included.