8VQ1

Pseudomonas fluorescens G150T isocyanide hydratase at 298 K XFEL data, thioimidate intermediate

Replaces:  8TSN

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Isonitrile hydratase InhA	polymer	231	24224.7	1	UniProt (Q4K977) Pfam (PF01965) In PDB	Pseudomonas fluorescens	Isocyanide hydratase
2	A	N-(4-nitrophenyl)methanimine	non-polymer		150.1	1	Chemie (QCV)
3	A	CHLORIDE ION	non-polymer		35.5	1	Chemie (CL)
4		water	water		18.0	154	Chemie (HOH)

Sequence modifications

A: 1 - 228 (UniProt: Q4K977)

PDB	External Database	Details
Gly -2	-	expression tag
Ser -1	-	expression tag
His 0	-	expression tag
Thr 150	Gly 150	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	24224.7
Non-Polymers*	Number of molecules	2
	Total formula weight	185.6
All*	Total formula weight	24410.3

*Water molecules are not included.