8VP9

Cryo-EM structure of the cysteine-free ABC transporter PCAT1 bound with ADP and Substrate

This is a non-PDB format compatible entry.

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	ABC-type bacteriocin transporter	polymer	750	83610.2	2	UniProt (A3DCU1) Pfam (PF03412) Pfam (PF00664) Pfam (PF00005)	Acetivibrio thermocellus ATCC 27405
2	C, D (C, D)	Bacteriocin-type signal sequence-containing protein	polymer	113	12725.5	2	UniProt (A3DCU2)	Acetivibrio thermocellus ATCC 27405
3	E, G (A, B)	ADENOSINE-5'-DIPHOSPHATE	non-polymer		427.2	2	Chemie (ADP)
4	F, H, I (A, B)	3-[oxidanyl-[2-(trimethyl-$l^{4}-azanyl)ethoxy]phosphoryl]oxypropyl hexadecanoate	non-polymer		480.6	3	Chemie (A1ACX)

Sequence modifications

A, B: 1 - 727 (UniProt: A3DCU1)

PDB	External Database	Details
Met -22	-	initiating methionine
Gly -21	-	expression tag
His -20	-	expression tag
His -19	-	expression tag
His -18	-	expression tag
His -17	-	expression tag
His -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
Ser -10	-	expression tag
Ser -9	-	expression tag
Gly -8	-	expression tag
His -7	-	expression tag
Ile -6	-	expression tag
Asp -5	-	expression tag
Asp -4	-	expression tag
Asp -3	-	expression tag
Asp -2	-	expression tag
Lys -1	-	expression tag
His 0	-	expression tag
Ser 12	Cys 12	engineered mutation
Ser 21	Cys 21	engineered mutation
Val 25	Cys 25	engineered mutation
Phe 129	Cys 129	engineered mutation
Ala 171	Cys 171	engineered mutation
Ala 581	Cys 581	engineered mutation
Ala 687	Cys 687	engineered mutation
Leu 713	Cys 713	engineered mutation

C, D: 1 - 90 (UniProt: A3DCU2)

PDB	External Database	Details
Met -22	-	initiating methionine
Gly -21	-	expression tag
His -20	-	expression tag
His -19	-	expression tag
His -18	-	expression tag
His -17	-	expression tag
His -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
Ser -10	-	expression tag
Ser -9	-	expression tag
Gly -8	-	expression tag
His -7	-	expression tag
Ile -6	-	expression tag
Asp -5	-	expression tag
Asp -4	-	expression tag
Asp -3	-	expression tag
Asp -2	-	expression tag
Lys -1	-	expression tag
His 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	192671.4
Non-Polymers*	Number of molecules	5
	Total formula weight	2296.3
All*	Total formula weight	194967.7

*Water molecules are not included.