8VP3

Cryo-EM structure of the ABC transporter PCAT1 bound with MgADP and Substrate

This is a non-PDB format compatible entry.

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, C (A, B)	ABC-type bacteriocin transporter	polymer	750	83688.5	2	UniProt (A3DCU1) Pfam (PF03412) Pfam (PF00664) Pfam (PF00005)	Acetivibrio thermocellus ATCC 27405
2	B, D (D, C)	Bacteriocin-type signal sequence-containing protein	polymer	113	12725.5	2	UniProt (A3DCU2)	Acetivibrio thermocellus ATCC 27405
3	E, H (A, B)	ADENOSINE-5'-DIPHOSPHATE	non-polymer		427.2	2	Chemie (ADP)
4	F, I (A, B)	MAGNESIUM ION	non-polymer		24.3	2	Chemie (MG)
5	G (A)	3-[oxidanyl-[2-(trimethyl-$l^{4}-azanyl)ethoxy]phosphoryl]oxypropyl hexadecanoate	non-polymer		480.6	1	Chemie (A1ACX)
6	J (B)	(2S)-3-(hexadecanoyloxy)-2-[(9Z)-octadec-9-enoyloxy]propyl 2-(trimethylammonio)ethyl phosphate	non-polymer		760.1	1	Chemie (POV)

Sequence modifications

A, B: 1 - 727 (UniProt: A3DCU1)

PDB	External Database	Details
Met -22	-	initiating methionine
Gly -21	-	expression tag
His -20	-	expression tag
His -19	-	expression tag
His -18	-	expression tag
His -17	-	expression tag
His -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
Ser -10	-	expression tag
Ser -9	-	expression tag
Gly -8	-	expression tag
His -7	-	expression tag
Ile -6	-	expression tag
Asp -5	-	expression tag
Asp -4	-	expression tag
Asp -3	-	expression tag
Asp -2	-	expression tag
Lys -1	-	expression tag
His 0	-	expression tag

D, C: 1 - 90 (UniProt: A3DCU2)

PDB	External Database	Details
Met -22	-	initiating methionine
Gly -21	-	expression tag
His -20	-	expression tag
His -19	-	expression tag
His -18	-	expression tag
His -17	-	expression tag
His -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
Ser -10	-	expression tag
Ser -9	-	expression tag
Gly -8	-	expression tag
His -7	-	expression tag
Ile -6	-	expression tag
Asp -5	-	expression tag
Asp -4	-	expression tag
Asp -3	-	expression tag
Asp -2	-	expression tag
Lys -1	-	expression tag
His 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	192828.0
Non-Polymers*	Number of molecules	6
	Total formula weight	2143.7
All*	Total formula weight	194971.7

*Water molecules are not included.