8VOX

Cryo-EM structure of the ABC transporter PCAT1 cysteine-free core bound with MgADP

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	ABC-type bacteriocin transporter	polymer	601	67078.0	2	UniProt (A3DCU1) Pfam (PF00664) Pfam (PF00005)	Acetivibrio thermocellus ATCC 27405
2	C, E (A, B)	ADENOSINE-5'-DIPHOSPHATE	non-polymer		427.2	2	Chemie (ADP)
3	D, F (A, B)	MAGNESIUM ION	non-polymer		24.3	2	Chemie (MG)

Sequence modifications

A, B: 151 - 727 (UniProt: A3DCU1)

PDB	External Database	Details
Met 127	-	initiating methionine
Gly 128	-	expression tag
His 129	-	expression tag
His 130	-	expression tag
His 131	-	expression tag
His 132	-	expression tag
His 133	-	expression tag
His 134	-	expression tag
His 135	-	expression tag
His 136	-	expression tag
His 137	-	expression tag
His 138	-	expression tag
Ser 139	-	expression tag
Ser 140	-	expression tag
Gly 141	-	expression tag
His 142	-	expression tag
Ile 143	-	expression tag
Asp 144	-	expression tag
Asp 145	-	expression tag
Asp 146	-	expression tag
Asp 147	-	expression tag
Lys 148	-	expression tag
His 149	-	expression tag
Met 150	-	expression tag
Ala 171	Cys 171	engineered mutation
Ala 581	Cys 581	engineered mutation
Leu 687	Cys 687	engineered mutation
Ala 713	Cys 713	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	134156.0
Non-Polymers*	Number of molecules	4
	Total formula weight	903.0
All*	Total formula weight	135059.0

*Water molecules are not included.