8VNR
Homing endonuclease H98A I-PpoI-DNA complex at pH6.0 (K+ MES) with 1 mM Mn2+ for 600s and then 100 mM imidazole for 15 hrs
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | C, D | DNA (5'-D(*TP*TP*GP*AP*CP*TP*CP*TP*CP*TP*TP*AP*AP*GP*AP*GP*AP*GP*TP*CP*A)-3') | polymer | 21 | 6437.2 | 2 | synthetic construct | ||
| 2 | A, B | Intron-encoded endonuclease I-PpoI | polymer | 162 | 17613.0 | 2 | UniProt (Q94702) In PDB | Physarum polycephalum | I-Ppo |
| 3 | C, D | SODIUM ION | non-polymer | 23.0 | 2 | Chemie (NA) | |||
| 4 | C, D, A, B | GLYCEROL | non-polymer | 92.1 | 5 | Chemie (GOL) | |||
| 5 | A, B | ZINC ION | non-polymer | 65.4 | 4 | Chemie (ZN) | |||
| 6 | water | water | 18.0 | 316 | Chemie (HOH) |
Sequence modifications
A, B: 2 - 163 (UniProt: Q94702)
| PDB | External Database | Details |
|---|---|---|
| Ala 98 | His 98 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 4 |
| Total formula weight | 48100.3 | |
| Non-Polymers* | Number of molecules | 11 |
| Total formula weight | 768.1 | |
| All* | Total formula weight | 48868.4 |
