8VJM

SpoIVFB(E44Q variant):pro-sigmaK complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, C (A, C)	Stage IV sporulation protein FB	polymer	314	36990.5	2	UniProt (P26937) Pfam (PF02163)	Bacillus subtilis subsp. subtilis str. 168
2	B, D (B, D)	RNA polymerase sigma-K factor	polymer	135	15089.5	2	UniProt (P12254) Pfam (PF04542)	Bacillus subtilis subsp. subtilis str. 168	Sigma-27,Stage IV sporulation protein CB
3	E, G (A)	Lauryl Maltose Neopentyl Glycol	non-polymer		1005.2	2	Chemie (LMN)
4	F (A)	DI-PALMITOYL-3-SN-PHOSPHATIDYLETHANOLAMINE	non-polymer		692.0	1	Chemie (PEF)

Sequence modifications

A, C: 1 - 288 (UniProt: P26937)

PDB	External Database	Details
Gln 44	Glu 44	engineered mutation
Lys 289	-	expression tag
Leu 290	-	expression tag
Asp 291	-	expression tag
Tyr 292	-	expression tag
Lys 293	-	expression tag
Asp 294	-	expression tag
Asp 295	-	expression tag
Asp 296	-	expression tag
Asp 297	-	expression tag
Lys 298	-	expression tag
Asp 299	-	expression tag
Tyr 300	-	expression tag
Lys 301	-	expression tag
Asp 302	-	expression tag
Asp 303	-	expression tag
Asp 304	-	expression tag
Asp 305	-	expression tag
Lys 306	-	expression tag
Leu 307	-	expression tag
Glu 308	-	expression tag
His 309	-	expression tag
His 310	-	expression tag
His 311	-	expression tag
His 312	-	expression tag
His 313	-	expression tag
His 314	-	expression tag

B, D: 1 - 115 (UniProt: P12254)

PDB	External Database	Details
Leu 116	-	expression tag
Met 117	-	expression tag
His 118	-	expression tag
Leu 119	-	expression tag
Arg 120	-	expression tag
Ala 121	-	expression tag
Leu 122	-	expression tag
Lys 123	-	expression tag
Lys 124	-	expression tag
Thr 125	-	expression tag
Lys 126	-	expression tag
Lys 127	-	expression tag
Gly 128	-	expression tag
Ser 129	-	expression tag
His 130	-	expression tag
His 131	-	expression tag
His 132	-	expression tag
His 133	-	expression tag
His 134	-	expression tag
His 135	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	104160.1
Non-Polymers*	Number of molecules	3
	Total formula weight	2702.3
All*	Total formula weight	106862.5

*Water molecules are not included.