8VIW
Cryo-EM structure of heparosan synthase 2 from Pasteurella multocida with polysaccharide in the GlcNAc-T active site
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B, C, D | Heparosan synthase B | polymer | 559 | 64548.3 | 4 | UniProt (Q5SGE1) Pfam (PF00535) In PDB | Pasteurella multocida | |
| 2 | E, F, G, H | beta-D-glucopyranuronic acid-(1-4)-2-deoxy-2-(sulfoamino)-alpha-D-glucopyranose-(1-4)-beta-D-glucopyranuronic acid-(1-4)-2-deoxy-2-(sulfoamino)-alpha-D-glucopyranose-(1-4)-beta-D-glucopyranuronic acid | branched | 1028.8 | 4 | In PDB GlyTouCan (G86319LQ) | |||
| 3 | A, B, C, D | MANGANESE (II) ION | non-polymer | 54.9 | 8 | Chemie (MN) | |||
| 4 | A, B, C, D | URIDINE-5'-DIPHOSPHATE | non-polymer | 404.2 | 8 | Chemie (UDP) | |||
| 5 | water | water | 18.0 | 4 | Chemie (HOH) |
Sequence modifications
A, B, C, D: 98 - 651 (UniProt: Q5SGE1)
| PDB | External Database | Details |
|---|---|---|
| Gly 93 | - | expression tag |
| Ser 94 | - | expression tag |
| Ala 95 | - | expression tag |
| Ala 96 | - | expression tag |
| Ala 97 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 4 |
| Total formula weight | 258193.1 | |
| Branched | Number of molecules | 4 |
| Total formula weight | 4115.3 | |
| Non-Polymers* | Number of molecules | 16 |
| Total formula weight | 3672.8 | |
| All* | Total formula weight | 265981.2 |
