8VIW
Cryo-EM structure of heparosan synthase 2 from Pasteurella multocida with polysaccharide in the GlcNAc-T active site
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B, C, D (A, B, C, D) | Heparosan synthase B | polymer | 559 | 64548.3 | 4 | UniProt (Q5SGE1) | Pasteurella multocida | |
| 2 | E, F, G, H (E, F, G, H) | beta-D-glucopyranuronic acid-(1-4)-2-deoxy-2-(sulfoamino)-alpha-D-glucopyranose-(1-4)-beta-D-glucopyranuronic acid-(1-4)-2-deoxy-2-(sulfoamino)-alpha-D-glucopyranose-(1-4)-beta-D-glucopyranuronic acid | branched | 1028.8 | 4 | In PDB GlyTouCan (G86319LQ) | |||
| 3 | I, K, M, O, Q... (A, B, C, D) | MANGANESE (II) ION | non-polymer | 54.9 | 8 | Chemie (MN) PubChem (27854) | |||
| 4 | J, L, N, P, R... (A, B, C, D) | URIDINE-5'-DIPHOSPHATE | non-polymer | 404.2 | 8 | Chemie (UDP) PubChem (1158) PubChem (6031) PubChem (3084486) PubChem (6101632) PubChem (6604175) PubChem (11811538) PubChem (12049241) PubChem (25323044) PubChem (25323048) PubChem (44298965) PubChem (57448939) PubChem (53486216) PubChem (60098857) PubChem (91746160) PubChem (92332560) PubChem (92332561) PubChem (92332562) PubChem (92332563) PubChem (93088131) PubChem (93630083) | |||
| 5 | AA, BA, Y, Z (C, D, A, B) | water | water | 18.0 | 4 | Chemie (HOH) |
Sequence modifications
A, B, C, D: 98 - 651 (UniProt: Q5SGE1)
| PDB | External Database | Details |
|---|---|---|
| Gly 93 | - | expression tag |
| Ser 94 | - | expression tag |
| Ala 95 | - | expression tag |
| Ala 96 | - | expression tag |
| Ala 97 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 4 |
| Total formula weight | 258193.1 | |
| Branched | Number of molecules | 4 |
| Total formula weight | 4115.3 | |
| Non-Polymers* | Number of molecules | 16 |
| Total formula weight | 3672.8 | |
| All* | Total formula weight | 265981.2 |
