8VI8

Engineered glutamine binding protein and a cobaloxime ligand - no GLN bound

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D, E...	Amino acid ABC transporter substrate-binding protein	polymer	229	25254.7	6	UniProt (C3THM2) Pfam (PF00497) In PDB	Escherichia coli	Glutamine ABC transporter substrate-binding protein GlnH,Glutamine ABC transporter substrate-bindnig protein,Glutamine ABC transporter,periplasmic glutamine-binding protein (TC 3.A.1.3.2),substrate-binding protein,Glutamine-binding periplasmic protein,Periplasmic glutamine-binding protein,Periplasmic glutamine-binding protein permease,Permease of periplasmic glutamine-binding protein
2	E, F, A, B, C...	AZIDOBIS (DIMETHYLGLYOXIMATO) PYRIDINECOBALT	non-polymer		438.3	6	Chemie (X5Z)
3	E, F, A, B, C...	SULFATE ION	non-polymer		96.1	12	Chemie (SO4)
4	E, F, A, B, C...	SODIUM ION	non-polymer		23.0	11	Chemie (NA)
5		water	water		18.0	50	Chemie (HOH)

Sequence modifications

A, B, C, D, E, F: 1 - 226 (UniProt: C3THM2)

PDB	External Database	Details
Ser -2	-	expression tag
Asn -1	-	expression tag
Ala 0	-	expression tag
Ile 23	Lys 45	engineered mutation
Cys 72	Thr 94	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	151527.9
Non-Polymers*	Number of molecules	29
	Total formula weight	4035.6
All*	Total formula weight	155563.6

*Water molecules are not included.