8VHQ

Crystal structure of E. coli class Ia ribonucleotide reductase alpha subunit W28A variant bound to dATP and ATP

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D (A, B, C, D)	Ribonucleoside-diphosphate reductase 1 subunit alpha	polymer	779	87682.0	4	UniProt (P00452) Pfam (PF03477) Pfam (PF00317) Pfam (PF02867)	Escherichia coli K-12	Protein B1,Ribonucleoside-diphosphate reductase 1 R1 subunit,Ribonucleotide reductase 1
2	E, H, J, M, O... (A, B, C, D)	2'-DEOXYADENOSINE 5'-TRIPHOSPHATE	non-polymer		491.2	8	Chemie (DTP)
3	F, I, K, N, P... (A, B, C, D)	MAGNESIUM ION	non-polymer		24.3	8	Chemie (MG)
4	G, L, Q, V (A, B, C, D)	ADENOSINE-5'-TRIPHOSPHATE	non-polymer		507.2	4	Chemie (ATP)

Sequence modifications

A, B, C, D: 1 - 760 (UniProt: P00452)

PDB	External Database	Details
Met -18	-	initiating methionine
Gly -17	-	expression tag
Ser -16	-	expression tag
Ser -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
His -9	-	expression tag
Ser -8	-	expression tag
Ser -7	-	expression tag
Gly -6	-	expression tag
Leu -5	-	expression tag
Val -4	-	expression tag
Pro -3	-	expression tag
Arg -2	-	expression tag
Gly -1	-	expression tag
Ser 0	-	expression tag
Ala 28	Trp 28	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	350728.0
Non-Polymers*	Number of molecules	20
	Total formula weight	6152.6
All*	Total formula weight	356880.7

*Water molecules are not included.