8VHP

Crystal structure of E. coli class Ia ribonucleotide reductase alpha subunit W28A variant bound to CDP and two molecules of ATP

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D, E... (A, B, C, D, E...)	Ribonucleoside-diphosphate reductase 1 subunit alpha	polymer	779	87682.0	8	UniProt (P00452) Pfam (PF03477) Pfam (PF00317) Pfam (PF02867)	Escherichia coli K-12	Protein B1,Ribonucleoside-diphosphate reductase 1 R1 subunit,Ribonucleotide reductase 1
2	AB, BB, CB, DB, EB... (D, E, B, C, G...)	SULFATE ION	non-polymer		96.1	40	Chemie (SO4)
3	AC, BA, CA, CC, DC... (G, B, E, C, H...)	ADENOSINE-5'-TRIPHOSPHATE	non-polymer		507.2	24	Chemie (ATP)
4	AA, BC, DA, EC, IB... (B, G, E, C, H...)	MAGNESIUM ION	non-polymer		24.3	16	Chemie (MG)
5	EA, FC, MB, OA, OC... (B, G, E, C, H...)	CYTIDINE-5'-DIPHOSPHATE	non-polymer		403.2	8	Chemie (CDP)
6	SC, TC, UC, VC, WC... (A, B, C, D, E...)	water	water		18.0	1222	Chemie (HOH)

Sequence modifications

A, B, C, D, E, F, G, H: 1 - 760 (UniProt: P00452)

PDB	External Database	Details
Met -18	-	initiating methionine
Gly -17	-	expression tag
Ser -16	-	expression tag
Ser -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
His -9	-	expression tag
Ser -8	-	expression tag
Ser -7	-	expression tag
Gly -6	-	expression tag
Leu -5	-	expression tag
Val -4	-	expression tag
Pro -3	-	expression tag
Arg -2	-	expression tag
Gly -1	-	expression tag
Ser 0	-	expression tag
Ala 28	Trp 28	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	8
	Total formula weight	701456.1
Non-Polymers*	Number of molecules	88
	Total formula weight	19629.2
All*	Total formula weight	721085.2

*Water molecules are not included.