8VHH

Engineered holo tryptophan synthase (Tm9D8*) derived from T. maritima TrpB

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Tryptophan synthase beta chain 1	polymer	397	43955.2	2	UniProt (P50909) Pfam (PF00291)	Thermotoga maritima
2	C, G (A, B)	PYRIDOXAL-5'-PHOSPHATE	non-polymer		247.1	2	Chemie (PLP)
3	D, H (A, B)	POTASSIUM ION	non-polymer		39.1	2	Chemie (K)
4	E, F, I (A, B)	PHOSPHATE ION	non-polymer		95.0	3	Chemie (PO4)
5	J, K (A, B)	water	water		18.0	94	Chemie (HOH)

Sequence modifications

A, B: 1 - 389 (UniProt: P50909)

PDB	External Database	Details
Gly 19	Pro 19	engineered mutation
Gly 30	Glu 30	engineered mutation
Val 69	Ile 69	engineered mutation
Leu 96	Lys 96	engineered mutation
Leu 140	Pro 140	engineered mutation
Asp 167	Asn 167	engineered mutation
Phe 184	Ile 184	engineered mutation
Pro 213	Leu 213	engineered mutation
Ser 228	Gly 228	engineered mutation
Ser 292	Thr 292	engineered mutation
Leu 390	-	expression tag
Glu 391	-	expression tag
His 392	-	expression tag
His 393	-	expression tag
His 394	-	expression tag
His 395	-	expression tag
His 396	-	expression tag
His 397	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	87910.3
Non-Polymers*	Number of molecules	7
	Total formula weight	857.4
All*	Total formula weight	88767.7

*Water molecules are not included.