8VHF
Cryo-EM structure of GPR119-Gs-Nb35 complex with small molecule agonist MBX-2982
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (B) | Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1, HiBiT | polymer | 371 | 40039.6 | 1 | UniProt (P62873) Pfam (PF00400) | Homo sapiens (human) | Transducin beta chain 1 |
| 2 | B (C) | Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 | polymer | 71 | 7861.1 | 1 | UniProt (P59768) Pfam (PF00631) | Homo sapiens (human) | G gamma-I |
| 3 | C (N) | Nanobody35 | polymer | 138 | 15140.7 | 1 | Lama glama | ||
| 4 | D (A) | Guanine nucleotide-binding protein G(s) subunit alpha isoforms short | polymer | 394 | 45683.4 | 1 | UniProt (P63092) Pfam (PF00503) | Homo sapiens (human) | Adenylate cyclase-stimulating G alpha protein |
| 5 | E (R) | Soluble cytochrome b562,Glucose-dependent insulinotropic receptor,LgBiT | polymer | 615 | 67778.5 | 1 | UniProt (P0ABE7) UniProt (Q8TDV5) Pfam (PF07361) Pfam (PF00001) | Escherichia coli | |
| 6 | F (R) | 2-[1-(5-ethylpyrimidin-2-yl)piperidin-4-yl]-4-[[4-(1,2,3,4-tetrazol-1-yl)phenoxy]methyl]-1,3-thiazole | non-polymer | 448.5 | 1 | Chemie (8VP) |
Sequence modifications
B: 13 - 351 (UniProt: P62873)
A: 1 - 394 (UniProt: P63092)
R: -109 - -4 (UniProt: P0ABE7)
R: 1 - 335 (UniProt: Q8TDV5)
R: 336 - 503 (PDB: 8VHF)
| PDB | External Database | Details |
|---|---|---|
| Gly 7 | - | linker |
| Pro 8 | - | linker |
| Gly 9 | - | linker |
| Ser 10 | - | linker |
| Ser 11 | - | linker |
| Gly 12 | - | linker |
| Gly 352 | - | linker |
| Ser 353 | - | linker |
| Ser 354 | - | linker |
| Gly 355 | - | linker |
| Gly 356 | - | linker |
| Gly 357 | - | linker |
| Gly 358 | - | linker |
| Ser 359 | - | linker |
| Gly 360 | - | linker |
| Gly 361 | - | linker |
| Gly 362 | - | linker |
| Gly 363 | - | linker |
| Ser 364 | - | linker |
| Ser 365 | - | linker |
| Gly 366 | - | linker |
| Val 367 | - | expression tag |
| Ser 368 | - | expression tag |
| Gly 369 | - | expression tag |
| Trp 370 | - | expression tag |
| Arg 371 | - | expression tag |
| Leu 372 | - | expression tag |
| Phe 373 | - | expression tag |
| Lys 374 | - | expression tag |
| Lys 375 | - | expression tag |
| Ile 376 | - | expression tag |
| Ser 377 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| Asn 54 | Ser 54 | engineered mutation |
| Ala 226 | Gly 226 | engineered mutation |
| Ala 268 | Glu 268 | engineered mutation |
| Lys 271 | Asn 271 | engineered mutation |
| Asp 274 | Lys 274 | engineered mutation |
| Lys 280 | Arg 280 | engineered mutation |
| Asp 284 | Thr 284 | engineered mutation |
| Thr 285 | Ile 285 | engineered mutation |
| PDB | External Database | Details |
|---|---|---|
| Pro -111 | - | linker |
| Gly -110 | - | linker |
| Trp -103 | Met 29 | engineered mutation |
| Ile -8 | His 124 | engineered mutation |
| Leu -4 | Arg 128 | engineered mutation |
| Gly -3 | - | linker |
| Ser -2 | - | linker |
| Pro -1 | - | linker |
| Ile 0 | - | linker |
R: 336 - 503 (PDB: 8VHF)
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 5 |
| Total formula weight | 176503.5 | |
| Non-Polymers* | Number of molecules | 1 |
| Total formula weight | 448.5 | |
| All* | Total formula weight | 176952.0 |
