8VGI

CryoEM structure of tryptase in complex with engineered conformationally rigid anti-tryptase Fab E104.v1.2DS

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D (A, B, C, D)	Tryptase alpha/beta-1	polymer	260	29211.1	4	UniProt (Q15661) Pfam (PF00089)	Homo sapiens (human)	Tryptase-1,Tryptase I,Tryptase alpha-1
2	E, G, I, L (E, G, I, L)	Fab E104.v1.2DS light chain	polymer	217	23623.3	4		Homo sapiens (human)
3	F, H, J, K (F, H, J, K)	Fab E104.v1.2DS heavy chain	polymer	237	25154.3	4		Homo sapiens (human)
4	M, N, O, P (A, B, C, D)	water	water		18.0	26	Chemie (HOH)

Sequence modifications

A, B, C, D: 16 - 246 (UniProt: Q15661)

PDB	External Database	Details
Ala 1	-	expression tag
Gly 2	-	expression tag
Ser 3	-	expression tag
Thr 4	-	expression tag
His 5	-	expression tag
His 6	-	expression tag
His 7	-	expression tag
His 8	-	expression tag
His 9	-	expression tag
His 10	-	expression tag
Asp 11	-	expression tag
Asp 12	-	expression tag
Asp 13	-	expression tag
Asp 14	-	expression tag
Lys 15	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	12
	Total formula weight	311954.5
All*	Total formula weight	311954.5

*Water molecules are not included.