8VF5

CryoEM structure of Ku homodimer in complex with linear DNA

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Non-homologous end joining protein Ku	polymer	295	33609.9	2	UniProt (P9WKD9) Pfam (PF02735)	Mycobacterium tuberculosis H37Rv	Mt-Ku
2	C (G)	DNA (40-MER)	polymer	40	12239.8	1		synthetic construct
3	D (H)	DNA (40-MER)	polymer	40	12392.0	1		synthetic construct

Sequence modifications

A, B: 1 - 273 (UniProt: P9WKD9)

PDB	External Database	Details
Met -21	-	initiating methionine
Gly -20	-	expression tag
His -19	-	expression tag
His -18	-	expression tag
His -17	-	expression tag
His -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
Ser -9	-	expression tag
Ser -8	-	expression tag
Gly -7	-	expression tag
His -6	-	expression tag
Ile -5	-	expression tag
Glu -4	-	expression tag
Gly -3	-	expression tag
Arg -2	-	expression tag
His -1	-	expression tag
Met 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	91851.5
All*	Total formula weight	91851.5

*Water molecules are not included.