8VEK
IsPETase - ACC mutant
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Poly(ethylene terephthalate) hydrolase | polymer | 272 | 28574.8 | 1 | UniProt (A0A0K8P6T7) Pfam (PF01738) | Piscinibacter sakaiensis | PET hydrolase,PETase,PET-digesting enzyme |
| 2 | B, C (A) | GLYCEROL | non-polymer | 92.1 | 2 | Chemie (GOL) | |||
| 3 | D, E (A) | SULFATE ION | non-polymer | 96.1 | 2 | Chemie (SO4) | |||
| 4 | F (A) | water | water | 18.0 | 328 | Chemie (HOH) |
Sequence modifications
A: 30 - 290 (UniProt: A0A0K8P6T7)
| PDB | External Database | Details |
|---|---|---|
| Met 27 | - | initiating methionine |
| Gly 28 | - | expression tag |
| Ser 29 | - | expression tag |
| Ala 186 | Asp 186 | engineered mutation |
| Cys 233 | Asn 233 | engineered mutation |
| Cys 282 | Ser 282 | engineered mutation |
| Ala 291 | - | expression tag |
| Ser 292 | - | expression tag |
| His 293 | - | expression tag |
| His 294 | - | expression tag |
| His 295 | - | expression tag |
| His 296 | - | expression tag |
| His 297 | - | expression tag |
| His 298 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 1 |
| Total formula weight | 28574.8 | |
| Non-Polymers* | Number of molecules | 4 |
| Total formula weight | 376.3 | |
| All* | Total formula weight | 28951.1 |
