8VEK

IsPETase - ACC mutant

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Poly(ethylene terephthalate) hydrolase	polymer	272	28574.8	1	UniProt (A0A0K8P6T7) Pfam (PF01738) In PDB	Piscinibacter sakaiensis	PET hydrolase,PETase,PET-digesting enzyme
2	A	GLYCEROL	non-polymer		92.1	2	Chemie (GOL)
3	A	SULFATE ION	non-polymer		96.1	2	Chemie (SO4)
4		water	water		18.0	328	Chemie (HOH)

Sequence modifications

A: 30 - 290 (UniProt: A0A0K8P6T7)

PDB	External Database	Details
Met 27	-	initiating methionine
Gly 28	-	expression tag
Ser 29	-	expression tag
Ala 186	Asp 186	engineered mutation
Cys 233	Asn 233	engineered mutation
Cys 282	Ser 282	engineered mutation
Ala 291	-	expression tag
Ser 292	-	expression tag
His 293	-	expression tag
His 294	-	expression tag
His 295	-	expression tag
His 296	-	expression tag
His 297	-	expression tag
His 298	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	28574.8
Non-Polymers*	Number of molecules	4
	Total formula weight	376.3
All*	Total formula weight	28951.1

*Water molecules are not included.