8VCT

CyoEM structure of the TnsC(1-503)-TnsD(1-318)-DNA complex in a 6:2:1 stoichiometry from E. coli Tn7 bound to ATPgS and ADP

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, E, F, G, H... (G, E, F, C, B...)	Transposon Tn7 transposition protein TnsC	polymer	523	59318.9	6	UniProt (P05846) Pfam (PF13401)	Escherichia coli	Protein E
2	B, I (X, Y)	Transposon Tn7 transposition protein TnsD	polymer	318	36636.0	2	UniProt (P13991) Pfam (PF06527) Pfam (PF15978)	Escherichia coli
3	C (H)	DNA (50-MER)	polymer	50	15438.9	1		Escherichia coli
4	D (I)	DNA (50-MER)	polymer	50	15366.8	1		Escherichia coli
5	K, R (G, C)	PHOSPHOTHIOPHOSPHORIC ACID-ADENYLATE ESTER	non-polymer		523.2	2	Chemie (AGS)
6	L, O, Q, S, U... (G, E, F, C, B...)	MAGNESIUM ION	non-polymer		24.3	6	Chemie (MG)
7	M, V (X, Y)	ZINC ION	non-polymer		65.4	2	Chemie (ZN)
8	N, P, T, W (E, F, B, A)	ADENOSINE-5'-DIPHOSPHATE	non-polymer		427.2	4	Chemie (ADP)

Sequence modifications

G, E, F, C, B, A: 1 - 503 (UniProt: P05846)

PDB	External Database	Details
Gly 2	Ser 2	engineered mutation
Pro 504	-	expression tag
Asn 505	-	expression tag
Ser 506	-	expression tag
Ser 507	-	expression tag
Ser 508	-	expression tag
Val 509	-	expression tag
Asp 510	-	expression tag
Lys 511	-	expression tag
Leu 512	-	expression tag
Ala 513	-	expression tag
Ala 514	-	expression tag
Ala 515	-	expression tag
Leu 516	-	expression tag
Glu 517	-	expression tag
His 518	-	expression tag
His 519	-	expression tag
His 520	-	expression tag
His 521	-	expression tag
His 522	-	expression tag
His 523	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	10
	Total formula weight	459991.3
Non-Polymers*	Number of molecules	14
	Total formula weight	3031.9
All*	Total formula weight	463023.3

*Water molecules are not included.