8VCN

GluER mutant - W66F F269Y Q293T F68Y T36E P263L

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	N-ethylmaleimide reductase	polymer	367	40126.0	1	UniProt (A1E8I9) Pfam (PF00724)	Gluconobacter oxydans	NADH oxidase
2	B, C, E (A)	ACETATE ION	non-polymer		59.0	3	Chemie (ACT)
3	D, F (A)	GLYCEROL	non-polymer		92.1	2	Chemie (GOL)
4	G (A)	FLAVIN MONONUCLEOTIDE	non-polymer		456.3	1	Chemie (FMN)
5	H (A)	water	water		18.0	443	Chemie (HOH)

Sequence modifications

A: 2 - 361 (UniProt: A1E8I9)

PDB	External Database	Details
Met -5	-	initiating methionine
His -4	-	expression tag
His -3	-	expression tag
His -2	-	expression tag
His -1	-	expression tag
His 0	-	expression tag
His 1	-	expression tag
Glu 36	Thr 36	engineered mutation
Phe 66	Trp 66	engineered mutation
Tyr 68	Phe 68	engineered mutation
Leu 263	Pro 263	engineered mutation
Tyr 269	Phe 269	engineered mutation
Thr 293	Gln 293	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	40126.0
Non-Polymers*	Number of molecules	6
	Total formula weight	817.7
All*	Total formula weight	40943.7

*Water molecules are not included.