8VCJ
CryoEM structure of the TnsC(1-503)-TnsD(1-318)-DNA complex in a 7:2:1 stoichiometry from E. coli Tn7 bound to ATPgS and ADP
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | A, B, C, D, E... | Transposon Tn7 transposition protein TnsC | polymer | 523 | 59318.9 | 7 | UniProt (P05846) Pfam (PF13401) In PDB | Escherichia coli | Protein E |
2 | Y, X | Transposon Tn7 transposition protein TnsD | polymer | 318 | 36636.0 | 2 | UniProt (P13991) Pfam (PF06527) Pfam (PF15978) In PDB | Escherichia coli | |
3 | H | DNA | polymer | 50 | 15438.9 | 1 | Escherichia coli | ||
4 | I | DNA | polymer | 50 | 15366.8 | 1 | Escherichia coli | ||
5 | A, B, D, E, F | ADENOSINE-5'-DIPHOSPHATE | non-polymer | 427.2 | 5 | Chemie (ADP) | |||
6 | A, B, C, D, E... | MAGNESIUM ION | non-polymer | 24.3 | 7 | Chemie (MG) | |||
7 | C, G | PHOSPHOTHIOPHOSPHORIC ACID-ADENYLATE ESTER | non-polymer | 523.2 | 2 | Chemie (AGS) | |||
8 | X, Y | ZINC ION | non-polymer | 65.4 | 2 | Chemie (ZN) |
Sequence modifications
A, B, C, D, E, F, G: 1 - 503 (UniProt: P05846)
PDB | External Database | Details |
---|---|---|
Gly 2 | Ser 2 | engineered mutation |
Pro 504 | - | expression tag |
Asn 505 | - | expression tag |
Ser 506 | - | expression tag |
Ser 507 | - | expression tag |
Ser 508 | - | expression tag |
Val 509 | - | expression tag |
Asp 510 | - | expression tag |
Lys 511 | - | expression tag |
Leu 512 | - | expression tag |
Ala 513 | - | expression tag |
Ala 514 | - | expression tag |
Ala 515 | - | expression tag |
Leu 516 | - | expression tag |
Glu 517 | - | expression tag |
His 518 | - | expression tag |
His 519 | - | expression tag |
His 520 | - | expression tag |
His 521 | - | expression tag |
His 522 | - | expression tag |
His 523 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 11 |
Total formula weight | 519310.3 | |
Non-Polymers* | Number of molecules | 16 |
Total formula weight | 3483.5 | |
All* | Total formula weight | 522793.7 |