8VB9
Kinetic intermediate states of HIV-1 RT DNA synthesis captured by cryo-EM
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | HIV-1 reverse transcriptase/ribonuclease H P66 subunit | polymer | 557 | 64104.5 | 1 | UniProt (P03366) Pfam (PF00078) Pfam (PF06817) Pfam (PF06815) Pfam (PF00075) | Human immunodeficiency virus 1 | |
| 2 | B (B) | HIV-1 reverse transcriptase P51 subunit | polymer | 444 | 51944.7 | 1 | UniProt (P03366) Pfam (PF00078) Pfam (PF06817) Pfam (PF06815) | Human immunodeficiency virus 1 | |
| 3 | C (F) | DNA (38-MER) | polymer | 38 | 11739.5 | 1 | synthetic construct | ||
| 4 | D, E (A) | MAGNESIUM ION | non-polymer | 24.3 | 2 | Chemie (MG) | |||
| 5 | F (A) | 2'-DEOXYADENOSINE 5'-TRIPHOSPHATE | non-polymer | 491.2 | 1 | Chemie (DTP) |
Sequence modifications
A: 1 - 555 (UniProt: P03366)
B: 1 - 428 (UniProt: P03366)
| PDB | External Database | Details |
|---|---|---|
| Met -1 | - | expression tag |
| Val 0 | - | expression tag |
| Ser 280 | Cys 879 | engineered mutation |
| Asn 498 | Asp 1097 | engineered mutation |
| PDB | External Database | Details |
|---|---|---|
| Met -15 | - | expression tag |
| Ala -14 | - | expression tag |
| His -13 | - | expression tag |
| His -12 | - | expression tag |
| His -11 | - | expression tag |
| His -10 | - | expression tag |
| His -9 | - | expression tag |
| His -8 | - | expression tag |
| Ala -7 | - | expression tag |
| Leu -6 | - | expression tag |
| Glu -5 | - | expression tag |
| Val -4 | - | expression tag |
| Leu -3 | - | expression tag |
| Phe -2 | - | expression tag |
| Gln -1 | - | expression tag |
| Gly 0 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 3 |
| Total formula weight | 127788.7 | |
| Non-Polymers* | Number of molecules | 3 |
| Total formula weight | 539.8 | |
| All* | Total formula weight | 128328.5 |
