8VB8

Kinetic intermediate states of HIV-1 RT DNA synthesis captured by cryo-EM

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	HIV-1 reverse transcriptase/ribonuclease H P66 subunit	polymer	557	64104.5	1	UniProt (P03366) Pfam (PF00078) Pfam (PF06817) Pfam (PF06815) Pfam (PF00075)	Human immunodeficiency virus 1
2	B (B)	HIV-1 reverse transcriptase P51 subunit	polymer	444	51944.7	1	UniProt (P03366) Pfam (PF00078) Pfam (PF06817) Pfam (PF06815)	Human immunodeficiency virus 1
3	C (F)	DNA (38-MER)	polymer	38	11739.5	1		synthetic construct
4	D (A)	2'-DEOXYADENOSINE 5'-TRIPHOSPHATE	non-polymer		491.2	1	Chemie (DTP)
5	E, F (A)	MAGNESIUM ION	non-polymer		24.3	2	Chemie (MG)

Sequence modifications

A: 1 - 555 (UniProt: P03366)

PDB	External Database	Details
Met -1	-	expression tag
Val 0	-	expression tag
Ser 280	Cys 879	engineered mutation
Asn 498	Asp 1097	engineered mutation

B: 1 - 428 (UniProt: P03366)

PDB	External Database	Details
Met -15	-	expression tag
Ala -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
His -9	-	expression tag
His -8	-	expression tag
Ala -7	-	expression tag
Leu -6	-	expression tag
Glu -5	-	expression tag
Val -4	-	expression tag
Leu -3	-	expression tag
Phe -2	-	expression tag
Gln -1	-	expression tag
Gly 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	3
	Total formula weight	127788.7
Non-Polymers*	Number of molecules	3
	Total formula weight	539.8
All*	Total formula weight	128328.5

*Water molecules are not included.