8VAT

Structure of the E. coli clamp loader bound to the beta clamp in a Open-RNAp/t conformation

This is a non-PDB format compatible entry.

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	DNA polymerase III subunit delta	polymer	343	38745.6	1	UniProt (P28630) Pfam (PF06144) Pfam (PF14840) In PDB	Escherichia coli
2	B, C, D	DNA polymerase III subunit tau	polymer	376	41803.2	3	UniProt (P06710) Pfam (PF13177) Pfam (PF22608) Pfam (PF12169) In PDB	Escherichia coli	DNA polymerase III subunit gamma
3	E	DNA polymerase III subunit delta'	polymer	337	37272.8	1	UniProt (P28631) Pfam (PF13177) Pfam (PF21500) Pfam (PF09115) In PDB	Escherichia coli
4	F, G	Beta sliding clamp	polymer	369	40922.8	2	UniProt (P0A988) Pfam (PF02767) Pfam (PF02768) Pfam (PF00712) In PDB	Escherichia coli
5	H	RNA (5'-R(P*AP*GP*UP*GP*GP*UP*GP*UP*CP*UP*G)-3')	polymer	11	3540.1	1		synthetic construct
6	I	DNA (5'-D(P*TP*TP*TP*TP*TP*TP*TP*CP*AP*GP*AP*CP*AP*CP*CP*AP*CP*TP*GP*C)-3')	polymer	30	9067.9	1		synthetic construct
7	B, C, D, E	ZINC ION	non-polymer		65.4	4	Chemie (ZN)
8	B, C, D	ADENOSINE-5'-DIPHOSPHATE	non-polymer		427.2	3	Chemie (ADP)
9	B, C, D	BERYLLIUM TRIFLUORIDE ION	non-polymer		66.0	3	Chemie (BEF)
10	B, C, D	MAGNESIUM ION	non-polymer		24.3	3	Chemie (MG)

Sequence modifications

B, C, D: 1 - 373 (UniProt: P06710)

PDB	External Database	Details
Gly -2	-	expression tag
Pro -1	-	expression tag
His 0	-	expression tag

E: 1 - 334 (UniProt: P28631)

PDB	External Database	Details
Gly -2	-	expression tag
Pro -1	-	expression tag
His 0	-	expression tag

F, G: 1 - 366 (UniProt: P0A988)

PDB	External Database	Details
Gly -2	-	expression tag
Pro -1	-	expression tag
His 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	9
	Total formula weight	295881.5
Non-Polymers*	Number of molecules	13
	Total formula weight	1814.2
All*	Total formula weight	297695.7

*Water molecules are not included.