8VAS

Structure of the E. coli clamp loader bound to the beta clamp in an Altered-Collar conformation

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	DNA polymerase III subunit delta	polymer	343	38745.6	1	UniProt (P28630) Pfam (PF06144) Pfam (PF14840)	Escherichia coli
2	B, C, D (B, C, D)	DNA polymerase III subunit tau	polymer	376	41803.2	3	UniProt (P06710) Pfam (PF13177) Pfam (PF22608) Pfam (PF12169)	Escherichia coli	DNA polymerase III subunit gamma
3	E (E)	DNA polymerase III subunit delta'	polymer	337	37272.8	1	UniProt (P28631) Pfam (PF13177) Pfam (PF21500) Pfam (PF09115)	Escherichia coli
4	F, G (F, G)	Beta sliding clamp	polymer	369	40922.8	2	UniProt (P0A988) Pfam (PF02767) Pfam (PF02768) Pfam (PF00712)	Escherichia coli
5	H (H)	DNA (5'-D(P*GP*CP*AP*GP*AP*CP*AP*CP*TP*AP*CP*GP*AP*GP*TP*AP*CP*AP*TP*A)-3')	polymer	20	6136.0	1		synthetic construct
6	I (I)	DNA (26-MER)	polymer	30	9172.9	1		synthetic construct
7	J, N, R, V (B, C, D, E)	ZINC ION	non-polymer		65.4	4	Chemie (ZN)
8	K, O, S (B, C, D)	ADENOSINE-5'-DIPHOSPHATE	non-polymer		427.2	3	Chemie (ADP)
9	L, P, T (B, C, D)	BERYLLIUM TRIFLUORIDE ION	non-polymer		66.0	3	Chemie (BEF)
10	M, Q, U (B, C, D)	MAGNESIUM ION	non-polymer		24.3	3	Chemie (MG)

Sequence modifications

B, C, D: 1 - 373 (UniProt: P06710)

PDB	External Database	Details
Gly -2	-	expression tag
Pro -1	-	expression tag
His 0	-	expression tag

E: 1 - 334 (UniProt: P28631)

PDB	External Database	Details
Gly -2	-	expression tag
Pro -1	-	expression tag
His 0	-	expression tag

F, G: 1 - 366 (UniProt: P0A988)

PDB	External Database	Details
Gly -2	-	expression tag
Pro -1	-	expression tag
His 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	9
	Total formula weight	298582.4
Non-Polymers*	Number of molecules	13
	Total formula weight	1814.2
All*	Total formula weight	300396.6

*Water molecules are not included.