8VAQ
Structure of the E. coli clamp loader bound to the beta clamp in a Closed-DNA1 conformation
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | A | DNA polymerase III subunit delta | polymer | 343 | 38745.6 | 1 | UniProt (P28630) Pfam (PF06144) Pfam (PF14840) In PDB | Escherichia coli | |
2 | B, C, D | DNA polymerase III subunit tau | polymer | 376 | 41803.2 | 3 | UniProt (P06710) Pfam (PF13177) Pfam (PF22608) Pfam (PF12169) In PDB | Escherichia coli | DNA polymerase III subunit gamma |
3 | E | DNA polymerase III subunit delta' | polymer | 337 | 37272.8 | 1 | UniProt (P28631) Pfam (PF13177) Pfam (PF21500) Pfam (PF09115) In PDB | Escherichia coli | |
4 | F, G | Beta sliding clamp | polymer | 369 | 40922.8 | 2 | UniProt (P0A988) Pfam (PF02767) Pfam (PF02768) Pfam (PF00712) In PDB | Escherichia coli | |
5 | H | DNA (5'-D(P*GP*CP*AP*GP*AP*CP*AP*CP*TP*AP*CP*GP*AP*GP*TP*AP*CP*AP*TP*A)-3') | polymer | 20 | 6136.0 | 1 | synthetic construct | ||
6 | I | DNA (27-MER) | polymer | 30 | 9172.9 | 1 | synthetic construct | ||
7 | B, C, D, E | ZINC ION | non-polymer | 65.4 | 4 | Chemie (ZN) | |||
8 | B, C, D | ADENOSINE-5'-DIPHOSPHATE | non-polymer | 427.2 | 3 | Chemie (ADP) | |||
9 | B, C, D | MAGNESIUM ION | non-polymer | 24.3 | 3 | Chemie (MG) | |||
10 | C, D | BERYLLIUM TRIFLUORIDE ION | non-polymer | 66.0 | 3 | Chemie (BEF) |
Sequence modifications
B, C, D: 1 - 373 (UniProt: P06710)
E: 1 - 334 (UniProt: P28631)
F, G: 1 - 366 (UniProt: P0A988)
PDB | External Database | Details |
---|---|---|
Gly -2 | - | expression tag |
Pro -1 | - | expression tag |
His 0 | - | expression tag |
PDB | External Database | Details |
---|---|---|
Gly -2 | - | expression tag |
Pro -1 | - | expression tag |
His 0 | - | expression tag |
PDB | External Database | Details |
---|---|---|
Gly -2 | - | expression tag |
Pro -1 | - | expression tag |
His 0 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 9 |
Total formula weight | 298582.4 | |
Non-Polymers* | Number of molecules | 13 |
Total formula weight | 1814.2 | |
All* | Total formula weight | 300396.6 |