8V93

Cryo-EM structure of E. coli FimH lectin domain bound to Fabs 329-2 and 454-3

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Fab 454-3 heavy chain	polymer	223	23940.6	1		Mus musculus
2	B (B)	Fab 329-2 heavy chain	polymer	226	24103.9	1		Mus musculus
3	C (C)	Fab 454-3 light chain	polymer	214	23512.8	1		Mus musculus
4	D (D)	Type 1 fimbrin D-mannose specific adhesin FimH, Donor strand complemented with FimG peptide 'triple mutant'	polymer	310	32118.6	1	UniProt (P08191) UniProt (P08190) Pfam (PF09160) Pfam (PF00419)	Escherichia coli	Protein FimH
5	E (L)	Fab 329-2 light chain	polymer	214	23611.0	1		Mus musculus

Sequence modifications

D: 1 - 279 (UniProt: P08191)

PDB	External Database	Details
Ser 7	Asn 28	engineered mutation
Ala 15	Gly 36	engineered mutation
Ala 16	Gly 37	engineered mutation
Ala 27	Val 48	engineered mutation
Ser 70	Asn 91	engineered mutation
Gln 228	Asn 249	engineered mutation
Gly 280	-	linker
Gly 281	-	linker
Ser 282	-	linker
Ser 283	-	linker
Gly 284	-	linker
Gly 285	-	linker
Gly 286	-	linker

D: 287 - 300 (UniProt: P08190)

PDB	External Database	Details
Gly 301	-	expression tag
Gly 302	-	expression tag
His 303	-	expression tag
His 304	-	expression tag
His 305	-	expression tag
His 306	-	expression tag
His 307	-	expression tag
His 308	-	expression tag
His 309	-	expression tag
His 310	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	5
	Total formula weight	127286.9
All*	Total formula weight	127286.9

*Water molecules are not included.