8V7B

Human DNA polymerase eta-DNA-dT primer araCTP insertion ternary complex at pH7.0 (K+ MES) with 1 Ca2+ ion

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	DNA polymerase eta	polymer	435	48617.7	1	UniProt (Q9Y253) Pfam (PF00817) Pfam (PF21704) Pfam (PF11799)	Homo sapiens (human)	RAD30 homolog A,Xeroderma pigmentosum variant type protein
2	B (T)	DNA (5'-D(*CP*AP*TP*GP*AP*TP*GP*AP*CP*GP*CP*T)-3')	polymer	12	3662.4	1		synthetic construct
3	C (P)	DNA (5'-D(*AP*GP*CP*GP*TP*CP*AP*T*())-3')	polymer	8	2426.6	1		synthetic construct
4	D, E, F (A)	GLYCEROL	non-polymer		92.1	3	Chemie (GOL) PubChem (753)
5	G (A)	4-amino-1-{5-O-[(S)-hydroxy{[(R)-hydroxy(phosphonooxy)phosphoryl]oxy}phosphoryl]-beta-D-arabinofuranosyl}pyrimidin-2(1H)-one	non-polymer		483.2	1	Chemie (HF4) PubChem (593) PubChem (6176) PubChem (25322920) PubChem (25322922) PubChem (177679034) PubChem (44119983) PubChem (25774) PubChem (53393603) PubChem (56845824) PubChem (167714) PubChem (57161090) PubChem (57411974) PubChem (58271893) PubChem (90230980) PubChem (90478950) PubChem (6602106) PubChem (92449054) PubChem (123755547) PubChem (11641562) PubChem (12049240) PubChem (12358859) PubChem (153426422) PubChem (171699329) PubChem (173381624)
6	H (A)	CALCIUM ION	non-polymer		40.1	1	Chemie (CA) PubChem (271)
7	I (A)	POTASSIUM ION	non-polymer		39.1	1	Chemie (K) PubChem (813)
8	J, K, L (A, T, P)	water	water		18.0	397	Chemie (HOH)

Sequence modifications

A: 1 - 432 (UniProt: Q9Y253)

PDB	External Database	Details
Gly -2	-	expression tag
Pro -1	-	expression tag
His 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	3
	Total formula weight	54706.7
Non-Polymers*	Number of molecules	6
	Total formula weight	838.6
All*	Total formula weight	55545.3

*Water molecules are not included.