8V64
RCK1-RCK2 double mutant of human Slo1 in presence of EDTA - resting VSD
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B, C, D (A, B, C, D) | Calcium-activated potassium channel subunit alpha-1 | polymer | 1065 | 119724.0 | 4 | UniProt (Q12791) Pfam (PF00520) Pfam (PF22614) Pfam (PF03493) Pfam (PF21014) | Homo sapiens (human) | BK channel,BKCA alpha,Calcium-activated potassium channel,subfamily M subunit alpha-1,K(VCA)alpha,KCa1.1,Maxi K channel,MaxiK,Slo-alpha,Slo1,Slowpoke homolog,Slo homolog,hSlo |
| 2 | AA, E, F, G, H... (A, C, B, D) | POTASSIUM ION | non-polymer | 39.1 | 9 | Chemie (K) PubChem (813) | |||
| 3 | BA, CA, DA, EA, FA... (B, A, D, C) | (2S)-3-(hexadecanoyloxy)-2-[(9Z)-octadec-9-enoyloxy]propyl 2-(trimethylammonio)ethyl phosphate | non-polymer | 760.1 | 24 | Chemie (POV) PubChem (65167) PubChem (5497103) PubChem (6436017) PubChem (6506401) PubChem (168614) PubChem (10908846) PubChem (6070971) | |||
| 4 | HA, OB, Q, YA (B, D, A, C) | CHOLESTEROL | non-polymer | 386.7 | 4 | Chemie (CLR) PubChem (16219173) PubChem (16758052) PubChem (304) PubChem (176980071) PubChem (191463) PubChem (368063) PubChem (369319) PubChem (18640088) PubChem (1715243) PubChem (40477506) PubChem (40557614) PubChem (42579431) PubChem (42579432) PubChem (1715244) PubChem (23423981) PubChem (45044259) PubChem (638019) PubChem (49853509) PubChem (50990013) PubChem (44654663) PubChem (52934537) PubChem (56621683) PubChem (1549081) PubChem (1553803) PubChem (46937002) PubChem (57915018) PubChem (57939063) PubChem (58428240) PubChem (58445498) PubChem (58663286) PubChem (59450329) PubChem (59628412) PubChem (59965215) PubChem (71234641) PubChem (59993755) PubChem (60001463) PubChem (66634651) PubChem (59274407) PubChem (5283629) PubChem (5284644) PubChem (68055877) PubChem (68166463) PubChem (68315613) PubChem (5315842) PubChem (70795838) PubChem (70797612) PubChem (70954610) PubChem (70957185) PubChem (71463909) PubChem (68311425) PubChem (68311398) PubChem (143173313) PubChem (177156123) PubChem (6419195) PubChem (5997) PubChem (6994560) PubChem (7055705) PubChem (7055706) PubChem (7059398) PubChem (7059400) PubChem (7059635) PubChem (7103033) PubChem (7182477) PubChem (7182478) PubChem (89951918) PubChem (90224410) PubChem (91298236) PubChem (9909206) PubChem (10045721) PubChem (71168115) PubChem (10134774) PubChem (10293553) PubChem (71173031) PubChem (11003580) PubChem (11025495) PubChem (11058155) PubChem (11463329) PubChem (1551269) PubChem (90070115) PubChem (11868624) PubChem (11868625) PubChem (11869366) PubChem (11869367) PubChem (11869368) PubChem (11869369) PubChem (12120344) PubChem (12156156) PubChem (12303135) PubChem (12303148) PubChem (12303156) PubChem (12303159) PubChem (12303160) PubChem (117965027) PubChem (6432564) PubChem (101624109) PubChem (6710666) PubChem (124146240) PubChem (7059399) PubChem (124897607) PubChem (124896959) PubChem (10861996) PubChem (132837164) PubChem (11834483) PubChem (11888915) PubChem (11888914) PubChem (124225449) PubChem (124429284) PubChem (124909223) PubChem (124909224) PubChem (124909225) PubChem (138967089) PubChem (139566851) PubChem (130459183) PubChem (131992107) PubChem (15939944) PubChem (139568882) PubChem (142983773) PubChem (144054483) PubChem (144209461) PubChem (144331236) PubChem (144808354) PubChem (145053568) PubChem (145157652) PubChem (145215073) PubChem (145230478) PubChem (146320991) PubChem (147857230) PubChem (154993519) PubChem (171828367) PubChem (173516975) PubChem (158603858) PubChem (162906452) PubChem (162906453) PubChem (162906454) PubChem (162906455) PubChem (162906456) PubChem (162906457) PubChem (163255768) PubChem (163389210) PubChem (164665303) PubChem (166553771) PubChem (166712004) PubChem (170116175) PubChem (171764280) PubChem (171817101) PubChem (171817104) PubChem (174285573) | |||
| 5 | AB, BB, CB, DB, EB... (C, B, D, A) | Digitonin | non-polymer | 1229.3 | 36 | Chemie (AJP) | |||
| 6 | AC, BC, CC, ZB (B, C, D, A) | water | water | 18.0 | 4 | Chemie (HOH) |
Sequence modifications
A, B, C, D: 1 - 1056 (UniProt: Q12791)
| PDB | External Database | Details |
|---|---|---|
| Ala 362 | Asp 427 | engineered mutation |
| Ala 367 | Asp 432 | engineered mutation |
| Ala 894 | Asp 959 | engineered mutation |
| Ala 895 | Asp 960 | engineered mutation |
| Ala 896 | Asp 961 | engineered mutation |
| Ala 897 | Asp 962 | engineered mutation |
| Ser 1057 | - | expression tag |
| Asn 1058 | - | expression tag |
| Ser 1059 | - | expression tag |
| Leu 1060 | - | expression tag |
| Glu 1061 | - | expression tag |
| Val 1062 | - | expression tag |
| Leu 1063 | - | expression tag |
| Phe 1064 | - | expression tag |
| Gln 1065 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 4 |
| Total formula weight | 478896.1 | |
| Non-Polymers* | Number of molecules | 73 |
| Total formula weight | 64395.6 | |
| All* | Total formula weight | 543291.6 |
