8V64

RCK1-RCK2 double mutant of human Slo1 in presence of EDTA - resting VSD

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D (A, B, C, D)	Calcium-activated potassium channel subunit alpha-1	polymer	1065	119724.0	4	UniProt (Q12791) Pfam (PF00520) Pfam (PF22614) Pfam (PF03493) Pfam (PF21014)	Homo sapiens (human)	BK channel,BKCA alpha,Calcium-activated potassium channel,subfamily M subunit alpha-1,K(VCA)alpha,KCa1.1,Maxi K channel,MaxiK,Slo-alpha,Slo1,Slowpoke homolog,Slo homolog,hSlo
2	AA, E, F, G, H... (A, C, B, D)	POTASSIUM ION	non-polymer		39.1	9	Chemie (K) PubChem (813)
3	BA, CA, DA, EA, FA... (B, A, D, C)	(2S)-3-(hexadecanoyloxy)-2-[(9Z)-octadec-9-enoyloxy]propyl 2-(trimethylammonio)ethyl phosphate	non-polymer		760.1	24	Chemie (POV)
4	HA, OB, Q, YA (B, D, A, C)	CHOLESTEROL	non-polymer		386.7	4	Chemie (CLR) PubChem (5997)
5	AB, BB, CB, DB, EB... (C, B, D, A)	Digitonin	non-polymer		1229.3	36	Chemie (AJP) PubChem (6474107)
6	AC, BC, CC, ZB (B, C, D, A)	water	water		18.0	4	Chemie (HOH)

Sequence modifications

A, B, C, D: 1 - 1056 (UniProt: Q12791)

PDB	External Database	Details
Ala 362	Asp 427	engineered mutation
Ala 367	Asp 432	engineered mutation
Ala 894	Asp 959	engineered mutation
Ala 895	Asp 960	engineered mutation
Ala 896	Asp 961	engineered mutation
Ala 897	Asp 962	engineered mutation
Ser 1057	-	expression tag
Asn 1058	-	expression tag
Ser 1059	-	expression tag
Leu 1060	-	expression tag
Glu 1061	-	expression tag
Val 1062	-	expression tag
Leu 1063	-	expression tag
Phe 1064	-	expression tag
Gln 1065	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	478896.1
Non-Polymers*	Number of molecules	73
	Total formula weight	64395.6
All*	Total formula weight	543291.6

*Water molecules are not included.