8V5P

Crystal Structure of the C-terminal domain of the VZV gE ectodomain in complex with the Fab of human antibody 5A2

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, D, G, J (A, D, G, C)	Envelope glycoprotein E	polymer	254	28010.8	4	UniProt (A0A1B1JEP3) Pfam (PF02480)	Human alphaherpesvirus 3 (Varicella-zoster virus)
2	B, E, H, K (H, E, J, F)	5A2 Fab heavy chain	polymer	242	26039.0	4		Homo sapiens (human)
3	C, F, I, L (L, Y, X, I)	5A2 Fab Light Chain	polymer	210	22474.0	4		Homo sapiens (human)

Sequence modifications

A, D, G, C: 301 - 516 (UniProt: A0A1B1JEP3)

PDB	External Database	Details
Met 271	-	initiating methionine
Gly 272	-	expression tag
Thr 273	-	expression tag
Val 274	-	expression tag
Asn 275	-	expression tag
Lys 276	-	expression tag
Pro 277	-	expression tag
Val 278	-	expression tag
Val 279	-	expression tag
Gly 280	-	expression tag
Val 281	-	expression tag
Leu 282	-	expression tag
Met 283	-	expression tag
Gly 284	-	expression tag
Phe 285	-	expression tag
Gly 286	-	expression tag
Ile 287	-	expression tag
Ile 288	-	expression tag
Thr 289	-	expression tag
Gly 290	-	expression tag
Thr 291	-	expression tag
Leu 292	-	expression tag
Arg 293	-	expression tag
Ile 294	-	expression tag
Thr 295	-	expression tag
Asn 296	-	expression tag
Pro 297	-	expression tag
Val 298	-	expression tag
Arg 299	-	expression tag
Ala 300	-	expression tag
Gly 517	-	expression tag
Ser 518	-	expression tag
His 519	-	expression tag
His 520	-	expression tag
His 521	-	expression tag
His 522	-	expression tag
His 523	-	expression tag
His 524	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	12
	Total formula weight	306095.6
All*	Total formula weight	306095.6

*Water molecules are not included.