8V55

Human mitochondrial DNA polymerase gamma bound to a replication fork in an open conformation

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	DNA polymerase subunit gamma-1	polymer	1229	138723.4	1	UniProt (P54098) Pfam (PF18136)	Homo sapiens (human)
2	B, C (B, C)	DNA polymerase subunit gamma-2, mitochondrial	polymer	474	53787.3	2	UniProt (Q9UHN1) Pfam (PF03129)	Homo sapiens (human)	DNA polymerase gamma accessory 55 kDa subunit,p55,Mitochondrial DNA polymerase accessory subunit,MtPolB,PolG-beta
3	D (P)	DNA primer chain	polymer	25	7595.9	1		Homo sapiens
4	E (T)	DNA template chain	polymer	44	13597.7	1		Homo sapiens

Sequence modifications

A: 26 - 1239 (UniProt: P54098)

PDB	External Database	Details
Met 11	-	initiating methionine
Gly 12	-	expression tag
Gly 13	-	expression tag
Ser 14	-	expression tag
His 15	-	expression tag
His 16	-	expression tag
His 17	-	expression tag
His 18	-	expression tag
His 19	-	expression tag
His 20	-	expression tag
Gly 21	-	expression tag
Ser 22	-	expression tag
Arg 23	-	expression tag
Phe 24	-	expression tag
Met 25	-	expression tag
Ala 198	Asp 198	engineered mutation
Ala 200	Glu 200	engineered mutation

B, C: 26 - 485 (UniProt: Q9UHN1)

PDB	External Database	Details
Met 12	-	initiating methionine
Ala 13	-	expression tag
Ser 14	-	expression tag
Arg 15	-	expression tag
Gly 16	-	expression tag
Ser 17	-	expression tag
His 18	-	expression tag
His 19	-	expression tag
His 20	-	expression tag
His 21	-	expression tag
His 22	-	expression tag
His 23	-	expression tag
Gly 24	-	expression tag
Ala 25	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	5
	Total formula weight	267491.6
All*	Total formula weight	267491.6

*Water molecules are not included.